PMID- 27341784 OWN - NLM STAT- MEDLINE DCOM- 20170320 LR - 20171116 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 92 DP - 2016 Nov TI - Exopolysaccharide from Trichoderma pseudokoningii promotes maturation of murine dendritic cells. PG - 1155-1161 LID - S0141-8130(16)30603-1 [pii] LID - 10.1016/j.ijbiomac.2016.06.064 [doi] AB - Dendritic cells (DCs) are the key regulators of immune responses. In this study, the effect of an exopolysaccharide (EPS) from the culture broth of Trichoderma pseudokoningii on the phenotypic and functional maturation of murine DCs and its underlying molecular mechanisms were investigated. It showed that EPS induced the morphological changes of DCs and the enhanced expression of DCs featured surface molecules CD11c, CD86, CD80 and major histocompatibility complex II (MHC-II). Flow cytometry analysis showed that the treatment with EPS could reduce FITC-dextran uptake by DCs. Sequentially, the results of ELISA indicated that EPS could increase the production of interleukin-12p70 (IL-12p70) in culture supernatant of DCs. Immunofluorescence staining and western blot analysis further revealed that EPS significantly prompted nuclear factor (NF)-kappaB subunit p65 translocation, IkappaB-alpha protein degradation, and p38 mitogen-activated protein kinase (MAPK) phosphorylation. And the production of IL-12p70 was significantly decreased in condition of the inhibition of p38 or NF-kappaB signaling pathway. These findings suggested that EPS could induce DCs maturation through both p38 MAPK and NF-kappaB signaling pathways. CI - Copyright (c) 2016 Elsevier B.V. All rights reserved. FAU - Xu, Yanghui AU - Xu Y AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; Department of Pharmacy, Anqing Hospital Affiliated to Anhui Medical University, Anqing 246000, China. FAU - Li, Jing AU - Li J AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; Department of Pharmacy, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an 237000, China. FAU - Ju, Jing AU - Ju J AD - Department of Pharmacy, Anqing Hospital Affiliated to Anhui Medical University, Anqing 246000, China. FAU - Shen, Bingxiang AU - Shen B AD - Department of Pharmacy, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an 237000, China. FAU - Chen, Guochuang AU - Chen G AD - School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. FAU - Qian, Wen AU - Qian W AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. FAU - Zhu, Lei AU - Zhu L AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. FAU - Lu, Jingbo AU - Lu J AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. FAU - Liu, Chunyan AU - Liu C AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. FAU - Qin, Guozheng AU - Qin G AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. FAU - Wang, Guodong AU - Wang G AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. Electronic address: guodong201@163.com. FAU - Chen, Kaoshan AU - Chen K AD - Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. Electronic address: ksc313@126.com. LA - eng PT - Journal Article DEP - 20160621 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (B7-1 Antigen) RN - 0 (B7-2 Antigen) RN - 0 (CD11c Antigen) RN - 0 (Cd86 protein, mouse) RN - 0 (Dextrans) RN - 0 (Fungal Polysaccharides) RN - 0 (Fungal Proteins) RN - 0 (Transcription Factor RelA) RN - 0 (fluorescein isothiocyanate dextran) RN - 187348-17-0 (Interleukin-12) RN - EC 2.7.11.10 (Chuk protein, mouse) RN - EC 2.7.11.10 (I-kappa B Kinase) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) RN - I223NX31W9 (Fluorescein-5-isothiocyanate) SB - IM MH - Animals MH - B7-1 Antigen/genetics/immunology MH - B7-2 Antigen/genetics/immunology MH - Biological Transport/drug effects MH - CD11c Antigen/genetics/immunology MH - Cell Differentiation/*drug effects MH - Cell Line MH - Dendritic Cells/cytology/*drug effects/immunology MH - Dextrans/metabolism MH - Fluorescein-5-isothiocyanate/analogs & derivatives/metabolism MH - Fungal Polysaccharides/isolation & purification/*pharmacology MH - Fungal Proteins/isolation & purification/*pharmacology MH - Gene Expression Regulation MH - I-kappa B Kinase/genetics/immunology MH - Interleukin-12/genetics/immunology MH - Mice MH - Phosphorylation/drug effects MH - Signal Transduction MH - Transcription Factor RelA/genetics/immunology MH - Trichoderma/*chemistry MH - p38 Mitogen-Activated Protein Kinases/genetics/immunology OTO - NOTNLM OT - Exopolysaccharide OT - Maturation OT - Murine dendritic cells OT - Nuclear factor-kappaB OT - Trichoderma pseudokoningii OT - p38 MAPK EDAT- 2016/06/28 06:00 MHDA- 2017/03/21 06:00 CRDT- 2016/06/26 06:00 PHST- 2016/04/29 00:00 [received] PHST- 2016/06/18 00:00 [revised] PHST- 2016/06/20 00:00 [accepted] PHST- 2016/06/28 06:00 [pubmed] PHST- 2017/03/21 06:00 [medline] PHST- 2016/06/26 06:00 [entrez] AID - S0141-8130(16)30603-1 [pii] AID - 10.1016/j.ijbiomac.2016.06.064 [doi] PST - ppublish SO - Int J Biol Macromol. 2016 Nov;92:1155-1161. doi: 10.1016/j.ijbiomac.2016.06.064. Epub 2016 Jun 21.