PMID- 27341996
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20180620
IS  - 2210-7762 (Print)
VI  - 209
IP  - 7-8
DP  - 2016 Jul-Aug
TI  - Chromosomal aberrations in childhood acute lymphoblastic leukemia: 15-year single 
      center experience.
PG  - 340-7
LID - S2210-7762(16)30204-6 [pii]
LID - 10.1016/j.cancergen.2016.06.004 [doi]
AB  - Genetic analysis of leukemic cells significantly impacts prognosis and treatment 
      stratification in childhood acute lymphoblastic leukemia (ALL). Our retrospective 
      single center study of 86 children with ALL enrolled into three consecutive 
      treatment protocols (ALL-BFM 90, ALL-BFM 95 and ALL IC-BFM 2002) between 1991 and 
      2007 demonstrates the importance of conventional cytogenetics and fluorescence in 
      situ hybridization (FISH). Cytogenetic and FISH examinations were performed 
      successfully in 82/86 (95.3%) patients and chromosomal changes were detected in 
      78 of the 82 (95.1%) patients: in 69/73 patients with B-cell precursor (BCP)-ALL 
      and in 9/9 patients with T-lineage ALL (T-ALL). The most frequent chromosomal 
      changes in subgroups divided according to WHO classification independent of 
      treatment protocol and leukemia subtype were hyperdiploidy in 36 patients (with 
      >/=50 chromosomes in 23 patients, with 47-49 chromosomes 13 patients) followed by 
      translocation t(12;21) with ETV6/RUNX1 fusion detected by FISH in 18 (22%) 
      patients. Additional changes were detected in 16/18 (88.8%) ETV6/RUNX1-positive 
      ALL patients with predominant deletion or rearrangement of untranslocated ETV6 
      allele. Unique aberrations were detected in 4 patients and dicentric chromosomes 
      in 8 patients, one with T-ALL. These results demonstrate that cytogenetics and 
      FISH successfully provided important prognostic information and revealed not only 
      recurrent but also new and rare rearrangements requiring further investigation in 
      terms of prognostic significance.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Jarosova, Marie
AU  - Jarosova M
AD  - Department of Hemato-Oncology, Faculty of Medicine and Dentistry, University 
      Hospital Olomouc, Palacky University Olomouc, 775 15, Czech Republic. Electronic 
      address: marie.jarosova@fnol.cz.
FAU - Volejnikova, Jana
AU  - Volejnikova J
AD  - Department of Pediatrics, Faculty of Medicine and Dentistry, University Hospital 
      Olomouc, Palacky University, Czech Republic; Institute of Molecular and 
      Translational Medicine, Faculty of Medicine and Dentistry, Palacky University 
      Olomouc, Czech Republic.
FAU - Porizkova, Ilona
AU  - Porizkova I
AD  - Department of Hemato-Oncology, Faculty of Medicine and Dentistry, University 
      Hospital Olomouc, Palacky University Olomouc, 775 15, Czech Republic.
FAU - Holzerova, Milena
AU  - Holzerova M
AD  - Department of Hemato-Oncology, Faculty of Medicine and Dentistry, University 
      Hospital Olomouc, Palacky University Olomouc, 775 15, Czech Republic.
FAU - Pospisilova, Dagmar
AU  - Pospisilova D
AD  - Department of Pediatrics, Faculty of Medicine and Dentistry, University Hospital 
      Olomouc, Palacky University, Czech Republic.
FAU - Novak, Zbynek
AU  - Novak Z
AD  - Department of Pediatrics, Faculty of Medicine and Dentistry, University Hospital 
      Olomouc, Palacky University, Czech Republic.
FAU - Vrbkova, Jana
AU  - Vrbkova J
AD  - Institute of Molecular and Translational Medicine, Faculty of Medicine and 
      Dentistry, Palacky University Olomouc, Czech Republic.
FAU - Mihal, Vladimir
AU  - Mihal V
AD  - Department of Pediatrics, Faculty of Medicine and Dentistry, University Hospital 
      Olomouc, Palacky University, Czech Republic; Institute of Molecular and 
      Translational Medicine, Faculty of Medicine and Dentistry, Palacky University 
      Olomouc, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160611
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Retrospective Studies
OTO - NOTNLM
OT  - Childhood acute lymphoblastic leukemia
OT  - chromosomal aberration
OT  - cytogenetics
OT  - dicentric chromosome
OT  - fluorescence in situ hybridization (FISH)
EDAT- 2016/06/28 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/06/26 06:00
PHST- 2016/02/11 00:00 [received]
PHST- 2016/06/03 00:00 [revised]
PHST- 2016/06/06 00:00 [accepted]
PHST- 2016/06/26 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
AID - S2210-7762(16)30204-6 [pii]
AID - 10.1016/j.cancergen.2016.06.004 [doi]
PST - ppublish
SO  - Cancer Genet. 2016 Jul-Aug;209(7-8):340-7. doi: 10.1016/j.cancergen.2016.06.004. 
      Epub 2016 Jun 11.