PMID- 27342098
OWN - NLM
STAT- MEDLINE
DCOM- 20160908
LR  - 20160625
IS  - 0236-6290 (Print)
IS  - 0236-6290 (Linking)
VI  - 64
IP  - 2
DP  - 2016 Jun
TI  - Immunological and biochemical characterisation of 7ap, a short protein translated 
      from an alternative frame of ORF7 of PRRSV.
PG  - 273-87
LID - 10.1556/004.2016.027 [doi]
AB  - Sequence analysis revealed a short alternative open reading frame (ORF) named 
      ORF7a within the nucleocapsid gene of genetically divergent porcine reproductive 
      and respiratory syndrome virus (PRRSV) genomes. Alignment of the corresponding 
      protein sequences (named 7ap) revealed substantial heterogeneity among 7aps of 
      different genotypes, though all of them are predicted to be positively charged. 
      Green fluorescent protein and FLAG fusion constructs of ORF7a of the 
      HU-14432/2011 PRRSV demonstrated that 7ap is expressed. 7ap of HU- 14432/2011 
      (Hu7ap) was synthesised chemically, and ELISA experiments revealed that Hu7ap 
      binds strongly to mammalian IgGs. Protein-protein gel retardation assays and 
      complement fixation inhibition suggest that 7aps bind to the CH2 domain of the 
      IgG(Fc) fragment. Cellular localisation and immunological characteristics of 
      PRRSV 7ap may indicate multiple functions including nuclear and cytoplasmic 
      over-tuning of normal cellular processes and immunosuppression.
FAU - Olasz, Ferenc
AU  - Olasz F
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research, 
      Hungarian Academy of Sciences , Budapest , Hungary.
FAU - Denes, Bela
AU  - Denes B
AD  - Veterinary Diagnostic Directorate, National Food Chain Safety Office , Budapest , 
      Hungary.
FAU - Balint, Adam
AU  - Balint A
AD  - Veterinary Diagnostic Directorate, National Food Chain Safety Office , Budapest , 
      Hungary.
FAU - Magyar, Tibor
AU  - Magyar T
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research, 
      Hungarian Academy of Sciences , Budapest , Hungary.
FAU - Belak, Sandor
AU  - Belak S
AD  - Department of Biomedical Sciences and Veterinary Public Health (BVF), Swedish 
      University of Agricultural Sciences (SLU) , Uppsala , Sweden.
FAU - Zadori, Zoltan
AU  - Zadori Z
AD  - Institute for Veterinary Medical Research, Centre for Agricultural Research, 
      Hungarian Academy of Sciences , Budapest , Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Vet Hung
JT  - Acta veterinaria Hungarica
JID - 8406376
RN  - 0 (Immunoglobulin G)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Affinity
MH  - Antibody Specificity
MH  - Cells, Cultured
MH  - Complement Fixation Tests
MH  - Erythrocytes/metabolism
MH  - Gene Expression Regulation, Viral/physiology
MH  - Immunoglobulin G/blood/immunology
MH  - Mammals
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - Sequence Alignment
MH  - Viral Proteins/genetics/immunology/*metabolism
OTO - NOTNLM
OT  - IgG binding
OT  - PRRSV
OT  - complement fixation inhibition
OT  - overlapping ORF
OT  - positive charged short peptide
EDAT- 2016/06/28 06:00
MHDA- 2016/09/09 06:00
CRDT- 2016/06/26 06:00
PHST- 2016/06/26 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/09/09 06:00 [medline]
AID - 10.1556/004.2016.027 [doi]
PST - ppublish
SO  - Acta Vet Hung. 2016 Jun;64(2):273-87. doi: 10.1556/004.2016.027.