PMID- 27343171
OWN - NLM
STAT- MEDLINE
DCOM- 20171207
LR  - 20180104
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 221
IP  - 11
DP  - 2016 Nov
TI  - The effect of Dermatophagoides pteronyssinus group 7 allergen (Der p 7) on 
      dendritic cells and its role in T cell polarization.
PG  - 1319-28
LID - S0171-2985(16)30053-5 [pii]
LID - 10.1016/j.imbio.2016.04.002 [doi]
AB  - The innate signaling pathway for Th2 immunity activated by inhaled allergens has 
      not been well defined. Dendritic cells (DCs) can use their innate 
      pattern-recognition Toll-like receptors and C-type lectin receptors to generate 
      innate immunity and influence adaptive responses. The aim of this study was to 
      investigate the glycoform of Dermatophagoides pteronyssinus group 7 allergen (Der 
      p 7) and its functional interaction with dendritic cell-specific intercellular 
      adhesion molecule-3-grabbing non-integrin (DC-SIGN). Both bone marrow-derived 
      dendritic cells (BMDCs) derived from mice and monocyte-derived DCs (MDDCs) 
      derived from human peripheral blood mononuclear cells (PBMCs) were used to 
      investigate the function of Der p 7. Dendritic cells derived from THP1 were used 
      to investigate the glycol form of Der p 7. Der p 7 interacted with DC-SIGN as 
      recognized by immunoprecipitation and glycoprotein staining, and its function was 
      partially inhibited by deglycosylation. When BMDCs were cultured with Der p 7, 
      the secretion of IL-6 and gene expressions of IL-6, OX40L and Jagged-1 were 
      increased. IL4(+)/CD4(+) T cells could be induced by Der p 7, however 
      IFN-gamma(+)/CD4(+) T cells were down-regulated. The effects of Der p 7 on DCs were 
      down-regulated in the presence of DC-SIGN or TLR4 antibodies and deglycosylation. 
      rDer p 7 enhanced the DC differentiation of CD80 and CD86. The effects of Der p 7 
      pulsed-MDDCs on IL4(+)/CD4(+) and IFN-gamma(+)/CD4(+) T-cell differentiation of human 
      PBMCs were significantly increased after Der p 7 stimulation and decreased by 
      DC-SIGN antibody inhibition. In conclusion, the Der p 7 is a glycoprotein and its 
      function was partially through glycan binding. BMDCs could be activated by Der p 
      7 through TLR4 and DC-SIGN, and followed by the expression of OX40 ligand (OX40L) 
      and Jagged-1, the expressions of IL4(+)/CD4(+) cells were enhanced. These 
      findings identify a previously unrecognized function of Der p 7 and establish a 
      link between innate TLR4/C-type lectin receptors and adaptive Th2 immunity.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Tsai, Jaw-Ji
AU  - Tsai JJ
AD  - Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, 
      Taichung Veterans General Hospital, Taichung, Taiwan; College of Life Sciences, 
      National Chung Hsing University, Taichung, Taiwan; Institute of Clinical 
      Medicine, National Yang Ming University, Taipei, Taiwan.
FAU - Wang, Hsin-Chieh
AU  - Wang HC
AD  - College of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
FAU - Chiu, Chih-Liang
AU  - Chiu CL
AD  - Department of Medical Research, Taichung Veterans General Hospital, Taichung, 
      Taiwan.
FAU - Liao, En-Chih
AU  - Liao EC
AD  - Department of Medicine, Mackay Medical College, New Taipei, Taiwan. Electronic 
      address: enchih@mmc.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160613
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Dermatophagoides pteronyssinus antigen p 7)
RN  - 0 (Jagged-1 Protein)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, OX40)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins/*immunology
MH  - Biomarkers
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Differentiation/genetics/immunology
MH  - Cell Line
MH  - Cytokines/genetics/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Dermatophagoides pteronyssinus/immunology
MH  - Humans
MH  - Jagged-1 Protein/genetics/metabolism
MH  - Lectins, C-Type/metabolism
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - Mice
MH  - Polysaccharides/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, OX40/genetics/metabolism
MH  - T-Lymphocytes/cytology/*immunology/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
OTO - NOTNLM
OT  - C-type lectin receptor
OT  - DC-SIGN
OT  - Dendritic cell
OT  - Der p 7
OT  - Dermatophagoides pteronyssinus
EDAT- 2016/06/28 06:00
MHDA- 2017/12/08 06:00
CRDT- 2016/06/26 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/03/10 00:00 [revised]
PHST- 2016/04/12 00:00 [accepted]
PHST- 2016/06/26 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2017/12/08 06:00 [medline]
AID - S0171-2985(16)30053-5 [pii]
AID - 10.1016/j.imbio.2016.04.002 [doi]
PST - ppublish
SO  - Immunobiology. 2016 Nov;221(11):1319-28. doi: 10.1016/j.imbio.2016.04.002. Epub 
      2016 Jun 13.