PMID- 27344110
OWN - NLM
STAT- MEDLINE
DCOM- 20161222
LR  - 20161230
IS  - 1618-0429 (Electronic)
IS  - 0932-4739 (Linking)
VI  - 56
DP  - 2016 Oct
TI  - A clinical Acanthamoeba isolate harboring two distinct bacterial endosymbionts.
PG  - 21-25
LID - S0932-4739(16)30021-9 [pii]
LID - 10.1016/j.ejop.2016.04.002 [doi]
AB  - Acanthamoebae feed on bacteria but are also frequent hosts of bacterial 
      symbionts. Here, we describe the stable co-occurrence of two symbionts, one 
      affiliated to the genus Parachlamydia and the other to the candidate genus 
      Paracaedibacter (Alphaproteobacteria), within a clinical isolate of Acanthamoeba 
      hatchetti genotype T4. We performed fluorescence in situ hybridization (FISH) and 
      transmission electron microscopy (TEM) to describe this symbiosis. Our study adds 
      to other reports of simultaneous co-occurrence of two symbionts within one 
      Acanthamoeba cell.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Muller, Anneliese
AU  - Muller A
AD  - Institute for Milk Hygiene, University of Veterinary Medicine Vienna, 
      Veterinaerplatz 1, 1210 Vienna, Austria.
FAU - Walochnik, Julia
AU  - Walochnik J
AD  - Institute of Specific Prophylaxis and Tropical Medicine, Medical University of 
      Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
FAU - Wagner, Martin
AU  - Wagner M
AD  - Institute for Milk Hygiene, University of Veterinary Medicine Vienna, 
      Veterinaerplatz 1, 1210 Vienna, Austria.
FAU - Schmitz-Esser, Stephan
AU  - Schmitz-Esser S
AD  - Institute for Milk Hygiene, University of Veterinary Medicine Vienna, 
      Veterinaerplatz 1, 1210 Vienna, Austria. Electronic address: sse@iastate.edu.
LA  - eng
PT  - Journal Article
DEP - 20160413
PL  - Germany
TA  - Eur J Protistol
JT  - European journal of protistology
JID - 8917383
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Acanthamoeba/isolation & purification/*microbiology/ultrastructure
MH  - Alphaproteobacteria/classification/genetics/*physiology/ultrastructure
MH  - Amebiasis/*parasitology
MH  - Chlamydiales/classification/genetics/*physiology/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratitis/*parasitology
MH  - Microscopy, Electron, Transmission
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Symbiosis
OTO - NOTNLM
OT  - Acanthamoeba
OT  - Intracellular bacteria
OT  - Paracaedibacter
OT  - Parachlamydia
OT  - Protozoa
OT  - Symbiosis
EDAT- 2016/06/28 06:00
MHDA- 2016/12/23 06:00
CRDT- 2016/06/27 06:00
PHST- 2016/03/02 00:00 [received]
PHST- 2016/04/01 00:00 [revised]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/12/23 06:00 [medline]
PHST- 2016/06/27 06:00 [entrez]
AID - S0932-4739(16)30021-9 [pii]
AID - 10.1016/j.ejop.2016.04.002 [doi]
PST - ppublish
SO  - Eur J Protistol. 2016 Oct;56:21-25. doi: 10.1016/j.ejop.2016.04.002. Epub 2016 
      Apr 13.