PMID- 27345475
OWN - NLM
STAT- MEDLINE
DCOM- 20170804
LR  - 20181202
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 127
IP  - 1
DP  - 2017 Jan
TI  - Mesenchymal stem cells have antifibrotic effects on transforming growth 
      factor-beta1-stimulated vocal fold fibroblasts.
PG  - E35-E41
LID - 10.1002/lary.26121 [doi]
AB  - OBJECTIVES/HYPOTHESIS: Mesenchymal stem cells (MSCs) hold therapeutic promise for 
      vocal fold scar, yet the precise mechanism(s) underlying tissue level changes 
      remain unclear. We hypothesize that MSCs interact with native fibroblasts to 
      favorably affect healing. Furthermore, we hypothesize that these interactions 
      vary based on MSC source. METHODS: Vocal fold fibroblasts (VFFs), adipose-derived 
      stem cells, and bone marrow-derived stem cells (BMSCs) were extracted from 
      Sprague-Dawley rats; and a coculture model was employed culturing VFFs +/- 
      transforming growth factor (TGF-beta1) (10 ng/mL) +/- MSCs. Monoculture MSCs were also 
      prepared as a control. Both extracellular matrix (ECM) and components of the 
      TGF-beta signaling pathway were analyzed via polymerase chain reaction and western 
      blotting. RESULTS: Significantly decreased TGF-beta1 mRNA and alpha-smooth muscle actin 
      protein was observed in VFFs in response to TGF-beta1 in the coculture with both 
      MSCs (P < 0.05, P < 0.01). BMSCs significantly downregulated collagen I (P < 
      0.05), collagen III (P < 0.05), Smad3 (P < 0.01), and TGF-beta1 receptor I (P < 
      0.01) mRNA in VFFs. Hyaluronic synthase-1 and 2 increased in cocultured BMSCs 
      when compared with monocultured BMSCs at baseline and in response to TGF-beta1 (P < 
      0.01). CONCLUSION: MSCs had a favorable effect on ECM regulation as well as 
      suppression of TGF-beta1 signaling in VFF. Bidirectional paracrine signaling was 
      also observed as VFFs altered ECM regulation in MSCs. These data provide insight 
      into the regenerative effects of MSCs and provide a foundation for clinical 
      application. LEVEL OF EVIDENCE: NA Laryngoscope, 127:E35-E41, 2017.
CI  - (c) 2016 The American Laryngological, Rhinological and Otological Society, Inc.
FAU - Hiwatashi, Nao
AU  - Hiwatashi N
AD  - NYU Voice Center, Department of Otolaryngology-Head and Neck Surgery, New York 
      University School of Medicine, New York, New York, U.S.A.
FAU - Bing, Renjie
AU  - Bing R
AD  - NYU Voice Center, Department of Otolaryngology-Head and Neck Surgery, New York 
      University School of Medicine, New York, New York, U.S.A.
FAU - Kraja, Iv
AU  - Kraja I
AD  - NYU Voice Center, Department of Otolaryngology-Head and Neck Surgery, New York 
      University School of Medicine, New York, New York, U.S.A.
FAU - Branski, Ryan C
AU  - Branski RC
AD  - NYU Voice Center, Department of Otolaryngology-Head and Neck Surgery, New York 
      University School of Medicine, New York, New York, U.S.A.
LA  - eng
GR  - R01 DC013277/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20160627
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation/drug effects
MH  - Coculture Techniques
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Fibroblasts/*metabolism
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Vocal Cords/*cytology/*metabolism
PMC - PMC5177483
MID - NIHMS786473
OTO - NOTNLM
OT  - TGF-beta
OT  - Vocal fold
OT  - extracellular matrix
OT  - fibrosis
OT  - mesenchymal stem cells
OT  - voice
EDAT- 2016/06/28 06:00
MHDA- 2017/08/05 06:00
PMCR- 2018/01/01
CRDT- 2016/06/28 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/04/25 00:00 [revised]
PHST- 2016/05/09 00:00 [accepted]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2017/08/05 06:00 [medline]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.1002/lary.26121 [doi]
PST - ppublish
SO  - Laryngoscope. 2017 Jan;127(1):E35-E41. doi: 10.1002/lary.26121. Epub 2016 Jun 27.