PMID- 27347061
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Jul
TI  - Bushen-Qiangdu-Zhilv decoction inhibits osteogenic differentiation of rat 
      fibroblasts by regulating connexin 43.
PG  - 347-353
AB  - Bushen-Qiangdu-Zhilv (BQZ) decoction is a traditional Chinese medicinal compound 
      widely used for treating ankylosing spondylitis (AS). However, the mechanisms 
      underlying effects of BQZ remain largely unknown. Osteoblast differentiation of 
      fibroblasts plays an important role in heterotopic ossification (HO) of AS, and 
      connexin 43 (Cx43) is crucially involved in the osteoblast differentiation of 
      fibroblasts. The aim of the present study was to evaluate the effects of BQZ on 
      the osteogenic differentiation of fibroblasts by regulating Cx43. Rat fibroblasts 
      were treated with freeze-dried powder of BQZ, in the presence or absence of 
      recombinant human bone morphogenetic protein-2 (rhBMP-2). MTS assays were 
      performed to examine the inhibitory effects of BQZ on fibroblast proliferation. 
      Western blot assays were conducted to detect the protein expression of 
      core-binding factor alpha 1 (Cbfalpha1), Cx43 and phosphorylated Cx43 (pCx43). BQZ 
      appeared to inhibit fibroblast proliferation in a dose-dependent manner. 
      Furthermore, the expression of Cbfalpha1 and Cx43/pCx43 was significantly suppressed 
      by BQZ, with or without rhBMP-2 stimulation. Therefore, the present results 
      indicate that BQZ may exert an anti-AS effect by suppressing the osteogenic 
      differentiation of fibroblasts via Cx43 regulation.
FAU - Zhou, Ying-Yan
AU  - Zhou YY
AD  - Department of Rheumatology, College of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510006, P.R. China.
FAU - Huang, Run-Yue
AU  - Huang RY
AD  - Department of Rheumatology, The Second Affiliated Hospital, Guangzhou University 
      of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China.
FAU - Lin, Jie-Hua
AU  - Lin JH
AD  - Department of Rheumatology, College of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510006, P.R. China.
FAU - Xu, Yong-Yue
AU  - Xu YY
AD  - Department of Rheumatology, College of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510006, P.R. China.
FAU - He, Xiao-Hong
AU  - He XH
AD  - Department of Rheumatology, The Second Affiliated Hospital, Guangzhou University 
      of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China.
FAU - He, Yi-Ting
AU  - He YT
AD  - Department of New Drug Development, The Second Affiliated Hospital, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160426
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4906848
OTO - NOTNLM
OT  - ankylosing spondylitis
OT  - connexin 43
OT  - fibroblasts
OT  - osteogenic differentiation
EDAT- 2016/06/28 06:00
MHDA- 2016/06/28 06:01
PMCR- 2016/04/26
CRDT- 2016/06/28 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2016/03/07 00:00 [accepted]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/06/28 06:01 [medline]
PHST- 2016/04/26 00:00 [pmc-release]
AID - ETM-0-0-3292 [pii]
AID - 10.3892/etm.2016.3292 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Jul;12(1):347-353. doi: 10.3892/etm.2016.3292. Epub 2016 Apr 
      26.