PMID- 27347112
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Jul
TI  - Specific protein 1 depletion attenuates glucose uptake and proliferation of human 
      glioma cells by regulating GLUT3 expression.
PG  - 125-131
AB  - It has been reported previously that the expression of glucose transporter member 
      3 (GLUT3) is increased in malignant glioma cells compared with normal glial 
      cells. However, the regulating mechanism that causes this phenomenon remains 
      unknown. The present study investigated the regulating role of transcription 
      factor specific protein 1 (Sp1) in GLUT3 expression in a human glioma cell line. 
      In the present study, Sp1 was identified to directly bind to the GLUT3 
      5'-untranslated region in human glioma U251 cells. Small interfering RNA- and the 
      Sp1-inhibitor mithramycin A-mediated Sp1 knockdown experiments revealed that Sp1 
      depletion decreased glucose uptake and inhibited cell growth and invasion of U251 
      cells by downregulating GLUT3 expression. Therefore Sp1 is an important positive 
      regulator for the expression of GLUT3 in human glioma cells, and may explain the 
      overexpression of GLUT3 in U251 cells. These results suggest that Sp1 may have a 
      role in glioma treatment.
FAU - Zheng, Chuanyi
AU  - Zheng C
AD  - Affiliated Bayi Brain Hospital, General Hospital of Beijing Military Region, 
      Southern Medical University, Beijing 100072, P.R. China; Department of 
      Neurosurgery, The Affiliated Hospital of Hainan Medical College, Haikou, Hainan 
      570102, P.R. China.
FAU - Yang, Kun
AU  - Yang K
AD  - Department of Neurosurgery, The Affiliated Hospital of Hainan Medical College, 
      Haikou, Hainan 570102, P.R. China.
FAU - Zhang, Maoying
AU  - Zhang M
AD  - Affiliated Bayi Brain Hospital, General Hospital of Beijing Military Region, 
      Southern Medical University, Beijing 100072, P.R. China; Department of 
      Neurosurgery, The First Affiliated Hospital, Jinan University, Guangzhou, 
      Guangdong 510610, P.R. China.
FAU - Zou, Mingming
AU  - Zou M
AD  - Affiliated Bayi Brain Hospital, General Hospital of Beijing Military Region, 
      Southern Medical University, Beijing 100072, P.R. China.
FAU - Bai, Enqi
AU  - Bai E
AD  - Department of Neurosurgery, The Affiliated Hospital of Hainan Medical College, 
      Haikou, Hainan 570102, P.R. China.
FAU - Ma, Quanhong
AU  - Ma Q
AD  - Jiangsu Key Laboratory of Translational Research and Therapy for 
      Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, 
      Jiangsu 215123, P.R. China.
FAU - Xu, Ruxiang
AU  - Xu R
AD  - Affiliated Bayi Brain Hospital, General Hospital of Beijing Military Region, 
      Southern Medical University, Beijing 100072, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160517
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4906678
OTO - NOTNLM
OT  - GLUT3
OT  - SP1
OT  - glucose uptake
OT  - malignant gliomas
EDAT- 2016/06/28 06:00
MHDA- 2016/06/28 06:01
PMCR- 2016/05/17
CRDT- 2016/06/28 06:00
PHST- 2014/12/31 00:00 [received]
PHST- 2016/04/18 00:00 [accepted]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/06/28 06:01 [medline]
PHST- 2016/05/17 00:00 [pmc-release]
AID - OL-0-0-4599 [pii]
AID - 10.3892/ol.2016.4599 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Jul;12(1):125-131. doi: 10.3892/ol.2016.4599. Epub 2016 May 17.