PMID- 27347122
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Jul
TI  - Prospective evaluation of FISH for detecting upper tract urothelial carcinoma in 
      voided urine specimens.
PG  - 183-188
AB  - The aim of the present study was to estimate the diagnostic value of fluorescence 
      in situ hybridization (FISH) analysis of tumor cells in voided urine specimens 
      for detecting upper tract urothelial carcinoma (UTUC). Cytology and FISH analyses 
      were conducted on voided urine collected in the morning from 125 patients with 
      suspected UTUC. During follow-up, ureteroscopy with biopsy and histopathology 
      were used to confirm the presence of tumors. The average follow-up time was 23.8 
      months (range, 6-36 months). A total of 8 patients who could not be contacted 
      until the last follow-up were excluded from the study. Of the remaining 117 
      patients, 19 were histologically demonstrated to have UTUC, of whom, 3 patients 
      had stage pTis disease, 6 had stage pTa disease, 5 had stage pT1 disease and 5 
      had stage pT2 disease (7 G1, 8 G2 and 4 G3). The overall sensitivity of FISH to 
      detect UTUCs in voided urine specimens was 84.21% (16/19), whereas that of 
      cytology was 42.11% (8/19) (P<0.05). The overall specificity of FISH to detect 
      UTUCs in voided urine specimens was 89.80% (88/98), compared with 94.90% (93/98) 
      of cytology (P>0.05). The positive predictive value (PPV) and negative predictive 
      value of FISH were 80.00% (16/20) and 97.78% (88/90), respectively, whereas those 
      of cytology were 100.00% (8/8) (P>0.05) and 90.29% (93/103) (P>0.05), 
      respectively. The present data indicated that FISH was a method capable of 
      detecting UTUCs in voided urine specimens with good sensitivity and specificity, 
      although it exhibited a high rate of false positivity and low PPV.
FAU - Yu, Qiubo
AU  - Yu Q
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Li, Yanan
AU  - Li Y
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Li, Gang
AU  - Li G
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Li, Tinghong
AU  - Li T
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Zeng, Han
AU  - Zeng H
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Yang, Zhu
AU  - Yang Z
AD  - Cell Genetics Laboratory, Molecular Medical Laboratory Center, Chongqing Medical 
      University, Chongqing 400016, P.R. China.
FAU - Wang, Deling
AU  - Wang D
AD  - Department of Urological Surgery, The First Affiliated Hospital, Chongqing 
      Medical University, Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160517
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4906663
OTO - NOTNLM
OT  - fluorescence in situ hybridization
OT  - prospective
OT  - upper tract urothelial carcinoma
OT  - voided urine specimen
EDAT- 2016/06/28 06:00
MHDA- 2016/06/28 06:01
PMCR- 2016/05/17
CRDT- 2016/06/28 06:00
PHST- 2015/03/16 00:00 [received]
PHST- 2016/03/22 00:00 [accepted]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/06/28 06:01 [medline]
PHST- 2016/05/17 00:00 [pmc-release]
AID - OL-0-0-4592 [pii]
AID - 10.3892/ol.2016.4592 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Jul;12(1):183-188. doi: 10.3892/ol.2016.4592. Epub 2016 May 17.