PMID- 27347159
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Jul
TI  - Gene expression profiling analysis of ovarian cancer.
PG  - 405-412
AB  - As a gynecological oncology, ovarian cancer has high incidence and mortality. To 
      study the mechanisms of ovarian cancer, the present study analyzed the GSE37582 
      microarray. GSE37582 was downloaded from Gene Expression Omnibus and included 
      data from 74 ovarian cancer cases and 47 healthy controls. The 
      differentially-expressed genes (DEGs) were screened using linear models for 
      microarray data package in R and were further screened for functional annotation. 
      Next, Gene Ontology and pathway enrichment analysis of the DEGs was conducted. 
      The interaction associations of the proteins encoded by the DEGs were searched 
      using the Search Tool for the Retrieval of Interacting Genes, and the 
      protein-protein interaction (PPI) network was visualized by Cytoscape. Moreover, 
      module analysis of the PPI network was performed using the BioNet analysis tool 
      in R. A total of 284 DEGs were screened, consisting of 145 upregulated genes and 
      139 downregulated genes. In particular, downregulated FBJ murine osteosarcoma 
      viral oncogene homolog (FOS) was an oncogene, while downregulated 
      cyclin-dependent kinase inhibitor 1A (CDKN1A) was a tumor suppressor gene and 
      upregulated cluster of differentiation 44 (CD44) was classed as an 'other' gene. 
      The enriched functions included collagen catabolic process, stress-activated 
      mitogen-activated protein kinases cascade and insulin receptor signaling pathway. 
      Meanwhile, FOS (degree, 15), CD44 (degree, 9), B-cell CLL/lymphoma 2 (BCL2; 
      degree, 7), CDKN1A (degree, 7) and matrix metallopeptidase 3 (MMP3; degree, 6) 
      had higher connectivity degrees in the PPI network for the DEGs. These genes may 
      be involved in ovarian cancer by interacting with other genes in the module of 
      the PPI network (e.g., BCL2-FOS, BCL2-CDKN1A, FOS-CDKN1A, FOS-CD44, MMP3-MMP7 and 
      MMP7-CD44). Overall, BCL2, FOS, CDKN1A, CD44, MMP3 and MMP7 may be correlated 
      with ovarian cancer.
FAU - Yin, Ji-Gang
AU  - Yin JG
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Liu, Xian-Ying
AU  - Liu XY
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Wang, Bin
AU  - Wang B
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Wang, Dan-Yang
AU  - Wang DY
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Wei, Man
AU  - Wei M
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Fang, Hua
AU  - Fang H
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
FAU - Xiang, Mei
AU  - Xiang M
AD  - Key Lab of Zoonosis Research, Ministry of Education, The Second Hospital of Jilin 
      University, Changchun, Jilin 130041, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160601
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4906568
OTO - NOTNLM
OT  - differentially-expressed genes
OT  - module analysis
OT  - ovarian cancer
OT  - protein-protein interaction network
EDAT- 2016/06/28 06:00
MHDA- 2016/06/28 06:01
PMCR- 2016/06/01
CRDT- 2016/06/28 06:00
PHST- 2015/03/08 00:00 [received]
PHST- 2016/03/18 00:00 [accepted]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/06/28 06:01 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - OL-0-0-4663 [pii]
AID - 10.3892/ol.2016.4663 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Jul;12(1):405-412. doi: 10.3892/ol.2016.4663. Epub 2016 Jun 1.