PMID- 27347196
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Jul
TI  - Speckle-type POZ (pox virus and zinc finger protein) protein gene deletion in 
      ovarian cancer: Fluorescence in situ hybridization analysis of a tissue 
      microarray.
PG  - 658-662
AB  - The aim of the present study was to investigate the status of speckle-type POZ 
      (pox virus and zinc finger protein) protein (SPOP) gene located on chromosome 
      17q21 in ovarian cancer (OC). The present study evaluated a tissue microarray, 
      which contained 90 samples of ovarian cancer and 10 samples of normal ovarian 
      tissue, using fluorescence in situ hybridization (FISH). FISH is a method where a 
      SPOP-specific DNA red fluorescence probe was used for the experimental group and 
      a centromere-specific DNA green fluorescence probe for chromosome 17 was used for 
      the control group. The present study demonstrated that a deletion of the SPOP 
      gene was observed in 52.27% (46/88) of the ovarian cancer tissues, but was not 
      identified in normal ovarian tissues. Simultaneously, monosomy 17 was frequently 
      identified in the ovarian cancer tissues, but not in the normal ovarian tissues. 
      Furthermore, the present data revealed that the ovarian cancer histological 
      subtype and grade were significantly associated with a deletion of the SPOP gene, 
      which was assessed by the appearance of monosomy 17 in the ovarian cancer 
      samples; the deletion of the SPOP gene was observed in a large proportion of 
      serous epithelial ovarian cancer (41/61; 67.21%), particularly in grade 3 (31/37; 
      83.78%). In conclusion, deletion of the SPOP gene on chromosome 17 in ovarian 
      cancer samples, which results from monosomy 17, indicates that the SPOP gene may 
      serve as a tumor suppressor gene in ovarian cancer.
FAU - Hu, Xiaoyu
AU  - Hu X
AD  - Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Yang, Zhu
AU  - Yang Z
AD  - Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Zeng, Manman
AU  - Zeng M
AD  - Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Liu, Y I
AU  - Liu YI
AD  - Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Yang, Xiaotao
AU  - Yang X
AD  - Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Li, Yanan
AU  - Li Y
AD  - Molecular Medical Laboratory, Chongqing Medical University, Chongqing 400016, 
      P.R. China.
FAU - Li, X U
AU  - Li XU
AD  - Molecular Medical Laboratory, Chongqing Medical University, Chongqing 400016, 
      P.R. China.
FAU - Yu, Qiubo
AU  - Yu Q
AD  - Molecular Medical Laboratory, Chongqing Medical University, Chongqing 400016, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160530
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4907299
OTO - NOTNLM
OT  - fluorescence in situ hybridization
OT  - ovarian cancer
OT  - speckle-type pox virus and zinc finger protein
OT  - tissue microarray
EDAT- 2016/06/28 06:00
MHDA- 2016/06/28 06:01
PMCR- 2016/05/30
CRDT- 2016/06/28 06:00
PHST- 2015/04/09 00:00 [received]
PHST- 2016/04/22 00:00 [accepted]
PHST- 2016/06/28 06:00 [entrez]
PHST- 2016/06/28 06:00 [pubmed]
PHST- 2016/06/28 06:01 [medline]
PHST- 2016/05/30 00:00 [pmc-release]
AID - OL-0-0-4643 [pii]
AID - 10.3892/ol.2016.4643 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Jul;12(1):658-662. doi: 10.3892/ol.2016.4643. Epub 2016 May 30.