PMID- 27349533
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20181113
IS  - 1348-4400 (Electronic)
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 62
IP  - 5
DP  - 2016 Oct 18
TI  - Immunohistochemical characterization of the arcuate kisspeptin/neurokinin 
      B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the 
      hypothalamus during the estrous cycle in heifers.
PG  - 471-477
AB  - Elucidating the physiological mechanisms that control reproduction is an obvious 
      strategy for improving the fertility of cattle and developing new agents to 
      control reproductive functions. The present study aimed to identify kisspeptin 
      neurons in the bovine hypothalamus, clarifying that a central mechanism is also 
      present in the cattle brain, as kisspeptin is known to play an important role in 
      the stimulation of gonadotropin-releasing hormone (GnRH)/gonadotropin secretion 
      in other mammals. To characterize kisspeptin neurons in the bovine hypothalamus, 
      the co-localizations of kisspeptin and neurokinin B (NKB) or kisspeptin and 
      dynorphin A (Dyn) were examined. Hypothalamic tissue was collected from Japanese 
      Black or Japanese Black x Holstein crossbred cows during the follicular and 
      luteal phases. Brain sections, including the arcuate nucleus (ARC) and the 
      preoptic area (POA), were dual immunostained with kisspeptin and either NKB or 
      Dyn. In the ARC, both NKB and Dyn were co-localized in kisspeptin neurons during 
      both the follicular and luteal phases, demonstrating the presence of 
      kisspeptin/NKB/Dyn-containing neurons, referred to as KNDy neurons, in cows. In 
      the POA, no co-localization of kisspeptin with either NKB or Dyn was detected. 
      Kisspeptin expression in the follicular phase was higher than that in the luteal 
      phase, suggesting that kisspeptin expression in the POA is positively controlled 
      by estrogen in cows. The kisspeptin neuronal populations in the ARC and POA 
      likely play important roles in regulating the GnRH pulse and surge, respectively, 
      in cows.
FAU - Hassaneen, A
AU  - Hassaneen A
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, 
      Japan.
FAU - Naniwa, Yousuke
AU  - Naniwa Y
FAU - Suetomi, Yuta
AU  - Suetomi Y
FAU - Matsuyama, Shuichi
AU  - Matsuyama S
FAU - Kimura, Koji
AU  - Kimura K
FAU - Ieda, Nahoko
AU  - Ieda N
FAU - Inoue, Naoko
AU  - Inoue N
FAU - Uenoyama, Yoshihisa
AU  - Uenoyama Y
FAU - Tsukamura, Hiroko
AU  - Tsukamura H
FAU - Maeda, Kei-Ichiro
AU  - Maeda KI
FAU - Matsuda, Fuko
AU  - Matsuda F
FAU - Ohkura, Satoshi
AU  - Ohkura S
LA  - eng
PT  - Journal Article
DEP - 20160627
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Kisspeptins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 74913-18-1 (Dynorphins)
RN  - 86933-75-7 (Neurokinin B)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Breeding
MH  - Cattle
MH  - Dynorphins/*metabolism
MH  - Estradiol/blood
MH  - Estrous Cycle/*physiology
MH  - Female
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Hypothalamus/*metabolism
MH  - Immunohistochemistry
MH  - Kisspeptins/*metabolism
MH  - Neurokinin B/*metabolism
MH  - Neurons/metabolism
MH  - Preoptic Area/metabolism
MH  - Progesterone/blood
MH  - Radioimmunoassay
PMC - PMC5081734
EDAT- 2016/10/21 06:00
MHDA- 2017/06/13 06:00
PMCR- 2016/10/01
CRDT- 2016/06/29 06:00
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/06/13 06:00 [medline]
PHST- 2016/06/29 06:00 [entrez]
PHST- 2016/10/01 00:00 [pmc-release]
AID - 2016-075 [pii]
AID - 10.1262/jrd.2016-075 [doi]
PST - ppublish
SO  - J Reprod Dev. 2016 Oct 18;62(5):471-477. doi: 10.1262/jrd.2016-075. Epub 2016 Jun 
      27.