PMID- 27350670 OWN - NLM STAT- MEDLINE DCOM- 20170328 LR - 20190923 IS - 0973-7731 (Electronic) IS - 0022-1333 (Linking) VI - 95 IP - 2 DP - 2016 Jun TI - Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum. PG - 283-9 AB - In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P <0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia. FAU - Zhu, Huaping AU - Zhu H AD - Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, People's Republic of China.zhhping2000@163.com. FAU - Liu, Zhigang AU - Liu Z FAU - Lu, Maixin AU - Lu M FAU - Gao, Fengying AU - Gao F FAU - Ke, Xiaoli AU - Ke X FAU - Ma, Dongmei AU - Ma D FAU - Huang, Zhanghan AU - Huang Z FAU - Cao, Jianmeng AU - Cao J FAU - Wang, Miao AU - Wang M LA - eng PT - Journal Article PL - India TA - J Genet JT - Journal of genetics JID - 2985113R RN - 0 (Genetic Markers) SB - IM MH - Alleles MH - Animals MH - Breeding MH - Chromosome Mapping MH - Chromosomes/*chemistry MH - Female MH - Genetic Linkage MH - Genetic Markers MH - Genotype MH - In Situ Hybridization, Fluorescence MH - Male MH - *Microsatellite Repeats MH - Phenotype MH - *Sex Determination Processes MH - Tilapia/*genetics EDAT- 2016/06/29 06:00 MHDA- 2017/03/30 06:00 CRDT- 2016/06/29 06:00 PHST- 2016/06/29 06:00 [entrez] PHST- 2016/06/29 06:00 [pubmed] PHST- 2017/03/30 06:00 [medline] AID - 10.1007/s12041-016-0653-y [doi] PST - ppublish SO - J Genet. 2016 Jun;95(2):283-9. doi: 10.1007/s12041-016-0653-y.