PMID- 27352316
OWN - NLM
STAT- MEDLINE
DCOM- 20170119
LR  - 20181202
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 73
IP  - 2
DP  - 2015 Nov
TI  - Experimental Research on Differentiation-Inducing Growth of Nerve Lateral Bud by 
      HUC-MSCs Chitosan Composite Conduit.
PG  - 305-311
LID - 10.1007/s12013-015-0578-8 [doi]
AB  - This study is intended to explore the role of human umbilical-cord-derived 
      mesenchymal stem cells (HUC-MSCs) in nerve end-to-side anastomosis, as well as in 
      the induction and promotion of growth of nerve lateral bud. The chitosan nerve 
      conduit was prepared based on the biological characteristics of chitosan, and the 
      nerve conduit was filled with HUC-MSCs, and was used to bridge the nerve 
      end-to-side anastomotic stoma. The experimental animals were randomly assigned 
      into three groups (10 in each group), and the nerve end-to-side anastomosis was 
      conducted: (1) group A (control group): traditional tibial nerve-common peroneal 
      nerve end-to-side anastomosis; (2) group B (experimental group 1): tibial 
      nerve-common peroneal nerve end-to-side anastomotic stoma bridged with chitosan 
      nerve conduit; (3) group C (experimental group 2): tibial nerve-common peroneal 
      nerve end-to-side anastomotic stoma bridged by chitosan nerve conduit filled with 
      HUC-MSCs. General morphological observation, nerve electrophysiology, and 
      anti-S-100 immunohistochemistry were performed. All experimental animals 
      survived, and no infections were found at operative incisions. The nerve 
      continuity was in good condition through visual observation when sampling, which 
      is mild adhesion to the surrounding tissue and easy to be separated. 12 W 
      HUC-MSCs chitosan composite nerve conduits were degraded completely after 
      operation. Electrophysiological test showed that the nerve conduction velocity 
      (NCV) in group C was significantly higher than that in group A or group B 
      (p < 0.01). There were no significant differences between NCVs of group A and 
      group B. Toluidine blue staining and transmission electron microscope showed that 
      the number of the medullated fibers and the myelin sheath thickness in group C 
      were larger than those in group A or B. There were no significant differences 
      between the numbers of the medullated fibers and between the myelin sheath 
      thicknesses of groups A and B. By means of anti-S-100 immunohistochemistry, the 
      arrangement of a large number of brown-red proliferating schwann cells around the 
      regenerated nerve fibers in group C could be found, while fewer and sparse 
      brown-red matters and very poor growth of schwann cells could be observed in 
      groups A and B. Slightly more favorable situation could be observed in group B 
      compared with group A. HUC-MSCs play obviously an important role in promoting 
      nerve regeneration during the nerve end-to-side anastomosis, which induces the 
      growth of axis bud, accelerates the growth velocity of regenerated fiber, and 
      promotes the growth and maturity of schwann cells.
FAU - Xiao, Qiang
AU  - Xiao Q
AD  - Department of Hand, Ankle and Foot Surgery, The First Affiliated Hospital of 
      Liaoning Medical University, Shenyang, 121001, Liaoning, China. 
      drxiaoqiang@163.com.
FAU - Zhang, Xuepu
AU  - Zhang X
AD  - Department of Hand, Ankle and Foot Surgery, The First Affiliated Hospital of 
      Liaoning Medical University, Shenyang, 121001, Liaoning, China.
FAU - Wu, Yuexin
AU  - Wu Y
AD  - Department of Hand, Ankle and Foot Surgery, The First Affiliated Hospital of 
      Liaoning Medical University, Shenyang, 121001, Liaoning, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (S100 Proteins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Microscopy, Electron, Transmission
MH  - Models, Animal
MH  - Nerve Regeneration
MH  - Rabbits
MH  - S100 Proteins/metabolism
MH  - Tibial Nerve/physiology
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Transplantation, Heterologous
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Chitosan composite nerve conduit
OT  - HUC-MSCs
OT  - Nerve end-to-side anastomosis
OT  - Nerve regeneration
EDAT- 2016/06/29 06:00
MHDA- 2017/01/20 06:00
CRDT- 2016/06/29 06:00
PHST- 2016/06/29 06:00 [entrez]
PHST- 2016/06/29 06:00 [pubmed]
PHST- 2017/01/20 06:00 [medline]
AID - 10.1007/s12013-015-0578-8 [pii]
AID - 10.1007/s12013-015-0578-8 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2015 Nov;73(2):305-311. doi: 10.1007/s12013-015-0578-8.