PMID- 27353768
OWN - NLM
STAT- MEDLINE
DCOM- 20170223
LR  - 20220408
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 146
IP  - 1
DP  - 2016 Jul
TI  - Bone Marrow Conventional Karyotyping and Fluorescence In Situ Hybridization: 
       Defining an Effective Utilization Strategy for Evaluation of Myelodysplastic 
      Syndromes.
PG  - 86-94
LID - 10.1093/ajcp/aqw077 [doi]
AB  - OBJECTIVES: The current standard of practice for evaluation of myelodysplastic 
      syndromes (MDS) includes peripheral blood and bone marrow morphology review and 
      conventional karyotyping. Karyotype provides a global view of the chromosome 
      complement while fluorescence in situ hybridization (FISH) targets specific 
      abnormalities. The aim of this study was to determine if an MDS-FISH panel would 
      add value beyond karyotype in MDS workup. METHODS: We studied 505 patients who 
      were evaluated for a possible MDS and had concurrent bone marrow examination, 
      karyotyping, and MDS-FISH performed. RESULTS: In total, 462 cases had adequate 
      karyotyping (>/=20 metaphases) and showed excellent concordance (96%, 445/462) 
      between karyotyping and MDS-FISH. Additional FISH abnormalities had no impact on 
      diagnosis and minimal impact on the cytogenetic prognostic scoring in the myeloid 
      neoplasm cases (2%, 4/206). The concordance rate dropped to 82% (32/39) in the 
      group with insufficient karyotyping (<20 metaphases), and additional FISH 
      findings in this subgroup had no impact on the diagnosis but altered the 
      cytogenetic prognostic scoring in 10% (2/20) of myeloid neoplasm cases. 
      CONCLUSIONS: In the evaluation of a possible MDS, FISH rarely provides additional 
      value when karyotype is adequate. We propose a value-based, cost-effective 
      algorithmic approach for conventional karyotyping and FISH testing in routine MDS 
      workup.
CI  - (c) American Society for Clinical Pathology, 2016. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - He, Rong
AU  - He R
AD  - From the Divisions of Hematopathology he.rong@mayo.edu.
FAU - Wiktor, Anne E
AU  - Wiktor AE
AD  - Laboratory Genetics.
FAU - Durnick, David K
AU  - Durnick DK
AD  - From the Divisions of Hematopathology.
FAU - Kurtin, Paul J
AU  - Kurtin PJ
AD  - From the Divisions of Hematopathology.
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
AD  - Laboratory Genetics.
FAU - Tefferi, Ayalew
AU  - Tefferi A
AD  - Hematology, Mayo Clinic College of Medicine, Rochester, MN.
FAU - Patnaik, Mrinal S
AU  - Patnaik MS
AD  - Hematology, Mayo Clinic College of Medicine, Rochester, MN.
FAU - Ketterling, Rhett P
AU  - Ketterling RP
AD  - From the Divisions of Hematopathology Laboratory Genetics.
FAU - Hanson, Curtis A
AU  - Hanson CA
AD  - From the Divisions of Hematopathology Laboratory Genetics.
LA  - eng
PT  - Journal Article
DEP - 20160627
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - Young Adult
OTO - NOTNLM
OT  - Genetics
OT  - Hematology
OT  - Hematopathology
OT  - Molecular diagnostics
EDAT- 2016/06/30 06:00
MHDA- 2017/02/24 06:00
CRDT- 2016/06/30 06:00
PHST- 2016/06/30 06:00 [entrez]
PHST- 2016/06/30 06:00 [pubmed]
PHST- 2017/02/24 06:00 [medline]
AID - aqw077 [pii]
AID - 10.1093/ajcp/aqw077 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2016 Jul;146(1):86-94. doi: 10.1093/ajcp/aqw077. Epub 2016 Jun 
      27.