PMID- 27355745
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20170330
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 31
IP  - 6
DP  - 2016 Jun
TI  - Neutrophil elastase ameliorates matrix metalloproteinase-9 to promote 
      lipopolysaccharide-induced acute lung injury in mice 1.
PG  - 382-8
LID - S0102-86502016000600382 [pii]
LID - 10.1590/S0102-865020160060000004 [doi]
AB  - PURPOSE: To investigate the regulatory roles of neutrophil elastase (NE) and 
      matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced acute lung 
      injury (ALI) in mice. METHODS: To construct LPS-induced ALI mouse models, 
      wild-type C57BL/6 mice were administered 5.0 mg/kg of LPS through endotracheal, 
      and/or 1.0 mg/kg of ONO-5046, and/or 20.0 mg/kg of chemically modified 
      tetracycline-3 (CMT-3) by gavage. The levels of MMP-9, tissue inhibitor of 
      metalloprotease-1, interleukin (IL)-6 were detected by real time RT-PCR at 6 h, 
      24 h and 48 h, and tumor necrosis factor (TNF), lung wet-dry weight ratio, white 
      blood cell (WBC) count and polymorphonuclear (PMN) count in bronchoalveolar 
      lavage fluid (BALF) were tested at 48 h after administration. The 5-day survival 
      analysis of the ALI mice was also performed. RESULTS: Both ONO-5046 and CMT-3, 
      regardless of being used individually or combined, significantly reduced the 
      levels of MMP-9, IL-6, and TNF in lung tissue as well as in BALF, and the WBC and 
      PMN count in BALF. Combined treatment with ONO-5046 and CMT-3 remarkably improved 
      the survival rate of ALI mice. CONCLUSION: Neutrophil elastase synergizes with 
      matrix metalloproteinase-9 to promote and regulate the release of inflammatory 
      mediators and the infiltration of inflammatory cells, consequently affecting the 
      survival of lipopolysaccharide-induced acute lung injury mice.
FAU - Guo, Yingfei
AU  - Guo Y
AD  - Department of Emergency, General Hospital of PLA, Beijing, China. Design the 
      protocol, acquisition and analysis of data, technical procedures, manuscript 
      writing, final approved.
AD  - Department of Emergency or Respiration, First Affiliated, General Hospital of 
      PLA, Beijing, China. Statistical analysis, technical procedures, final approved 
      the manuscript.
FAU - Ma, Lingyun
AU  - Ma L
AD  - Department of Emergency, General Hospital of PLA, Beijing, China. Design the 
      protocol, acquisition and analysis of data, technical procedures, manuscript 
      writing, final approved.
AD  - Department of Emergency or Respiration, First Affiliated, General Hospital of 
      PLA, Beijing, China. Statistical analysis, technical procedures, final approved 
      the manuscript.
FAU - Zhang, Fei
AU  - Zhang F
AD  - Department of Emergency, General Hospital of PLA, Beijing, China. Design the 
      protocol, acquisition and analysis of data, technical procedures, manuscript 
      writing, final approved.
FAU - Sun, Rongju
AU  - Sun R
AD  - Department of Emergency, General Hospital of PLA, Beijing, China. Design the 
      protocol, acquisition and analysis of data, technical procedures, manuscript 
      writing, final approved.
FAU - Li, Tanshi
AU  - Li T
AD  - Department of Emergency, General Hospital of PLA, Beijing, China. Design the 
      protocol, acquisition and analysis of data, technical procedures, manuscript 
      writing, final approved.
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Sulfonamides)
RN  - 0 (Tetracyclines)
RN  - 0 (Timp1 protein, mouse)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tumor Necrosis Factors)
RN  - 0 (interleukin-6, mouse)
RN  - 0 (tetracycline CMT-3)
RN  - DWI62G0P59 (sivelestat)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Acute Lung Injury/blood/chemically induced/*enzymology
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Disease Models, Animal
MH  - Glycine/administration & dosage/*analogs & derivatives
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-6/metabolism
MH  - Leukocyte Count
MH  - Leukocyte Elastase/drug effects/*metabolism
MH  - Lipopolysaccharides
MH  - Matrix Metalloproteinase 9/analysis/drug effects/*metabolism
MH  - Mice, Inbred C57BL
MH  - Neutrophils
MH  - Sulfonamides/*administration & dosage
MH  - Survival Analysis
MH  - Tetracyclines/*administration & dosage
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tumor Necrosis Factors/metabolism
EDAT- 2016/06/30 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/06/30 06:00
PHST- 2016/02/24 00:00 [received]
PHST- 2016/05/23 00:00 [accepted]
PHST- 2016/06/30 06:00 [entrez]
PHST- 2016/06/30 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
AID - S0102-86502016000600382 [pii]
AID - 10.1590/S0102-865020160060000004 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2016 Jun;31(6):382-8. doi: 10.1590/S0102-865020160060000004.