PMID- 27356856
OWN - NLM
STAT- MEDLINE
DCOM- 20170302
LR  - 20190320
IS  - 1881-7122 (Electronic)
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 65
IP  - 4
DP  - 2016 Nov 1
TI  - In situ hybridization study of CYP2D mRNA in the common marmoset brain.
PG  - 465-471
AB  - The common marmoset is a non-human primate that has increasingly employed in the 
      biomedical research including the fields of neuroscience and behavioral studies. 
      Cytochrome P450 (CYP) 2D has been speculated to be involved in psycho-neurologic 
      actions in the human brain. In the present study, to clarify the role of CYP2D in 
      the marmoset brain, we investigated the expression patterns of CYP2D mRNA in the 
      brain using in situ hybridization (ISH). In addition, to identify the gene 
      location of CYP2D19, a well-studied CYP2D isoform in the common marmoset, a 
      fluorescence in situ hybridization (FISH) study was performed. Consistent with 
      findings for the human brain, CYP2D mRNA was localized in the neuronal cells of 
      different brain regions; e.g., the cerebral cortex, hippocampus, substantia 
      nigra, and cerebellum. FISH analysis showed that the CYP2D19 gene was located on 
      chromosome 1q, which is homologous to human chromosome 22 on which the CYP2D6 
      gene exists. These results suggest that CYP2D in the marmoset brain may play the 
      same role as human CYP2D6 in terms of brain actions, and that the CYP2D19 gene is 
      conserved in a syntenic manner. Taken together, these findings suggest that the 
      common marmoset is a useful model for studying psychiatric disorders related to 
      CYP2D dysfunction in the brain.
FAU - Shimamoto, Yoshinori
AU  - Shimamoto Y
AD  - Laboratory of Animal Therapeutics, Department of Veterinary Science, Rakuno 
      Gakuen University, 582 Midorimachi, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
FAU - Niimi, Kimie
AU  - Niimi K
FAU - Kitamura, Hiroshi
AU  - Kitamura H
FAU - Tsubakishita, Sae
AU  - Tsubakishita S
FAU - Takahashi, Eiki
AU  - Takahashi E
LA  - eng
PT  - Journal Article
DEP - 20160629
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (RNA, Messenger)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.14.14.1 (cytochrome P-450 CYP2D19 (marmoset))
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/*genetics/metabolism
MH  - Brain/metabolism
MH  - Callithrix/*growth & development/metabolism
MH  - Cytochrome P-450 Enzyme System/*genetics/metabolism
MH  - *Gene Expression
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - RNA, Messenger/metabolism
PMC - PMC5111850
EDAT- 2016/11/03 06:00
MHDA- 2019/03/21 06:00
PMCR- 2016/01/01
CRDT- 2016/07/01 06:00
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2016/07/01 06:00 [entrez]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 16-0045 [pii]
AID - 10.1538/expanim.16-0045 [doi]
PST - ppublish
SO  - Exp Anim. 2016 Nov 1;65(4):465-471. doi: 10.1538/expanim.16-0045. Epub 2016 Jun 
      29.