PMID- 27357630
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 2
DP  - 2016 Aug
TI  - Contribution of human osteoblasts and macrophages to bone matrix degradation and 
      proinflammatory cytokine release after exposure to abrasive endoprosthetic wear 
      particles.
PG  - 1491-500
LID - 10.3892/mmr.2016.5415 [doi]
AB  - One of the major reasons for failure after total joint arthroplasty is aseptic 
      loosening of the implant. At articulating surfaces, defined as the interface 
      between implant and surrounding bone cement, wear particles can be generated and 
      released into the periprosthetic tissue, resulting in inflammation and 
      osteolysis. The aim of the present study was to evaluate the extent to which 
      osteoblasts and macrophages are responsible for the osteolytic and inflammatory 
      reactions following contact with generated wear particles from Ti‑6Al‑7Nb and 
      Co‑28Cr‑6Mo hip stems. To this end, human osteoblasts and THP‑1 monocytic cells 
      were incubated with the experimentally generated wear particles as well as 
      reference particles (0.01 and 0.1 mg/ml) for 48 h under standard culture 
      conditions. To evaluate the impact of these particles on the two cell types, the 
      release of different bone matrix degrading matrix metalloproteinases (MMPs), 
      tissue inhibitors of MMPs (TIMPs), and relevant cytokines were determined by 
      multiplex enzyme‑linked immunosorbent assays. Following incubation with wear 
      particles, human osteoblasts showed a significant upregulation of MMP1 and MMP8, 
      whereas macrophages reacted with enhanced MMP3, MMP8 and MMP10 production. 
      Moreover, the synthesis of TIMPs 1 and 2 was inhibited. The osteoblasts and 
      macrophages also responded with modified expression of the inflammatory mediators 
      interleukin (IL)‑6, IL‑8, monocyte chemoattractant protein‑1 and vascular 
      endothelial growth factor. These results demonstrate that the release of wear 
      particles affects the release of proinflammatory cytokines and has a negative 
      impact on bone matrix formation during the first 48 h of particle exposure. Human 
      osteoblasts are directly involved in the proinflammatory cascade of bone matrix 
      degradation. The simultaneous activation and recruitment of monocytes/macrophages 
      boosted osteolytic processes in the periprosthetic tissue. By the downregulation 
      of TIMP production and the concomitant upregulation of MMPs as a response to 
      particle exposure, bone formation around implants may be suppressed, resulting in 
      implant failure.
FAU - Jonitz-Heincke, Anika
AU  - Jonitz-Heincke A
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
FAU - Lochner, Katrin
AU  - Lochner K
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
FAU - Schulze, Christoph
AU  - Schulze C
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
FAU - Pohle, Diana
AU  - Pohle D
AD  - Department of Immunology, University Medical Center Rostock, Rostock 18057, 
      Germany.
FAU - Pustlauk, Wera
AU  - Pustlauk W
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
FAU - Hansmann, Doris
AU  - Hansmann D
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
FAU - Bader, Rainer
AU  - Bader R
AD  - Department of Orthopedics, Biomechanics and Implant Technology Research 
      Laboratory, University Medical Center Rostock, Rostock 18057, Germany.
LA  - eng
PT  - Journal Article
DEP - 20160621
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Arthroplasty, Replacement/adverse effects
MH  - Bone Matrix/*metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Inflammation Mediators/*metabolism
MH  - Macrophages/*metabolism
MH  - Matrix Metalloproteinases/genetics/metabolism
MH  - Osteoblasts/*metabolism
MH  - Osteolysis/metabolism
MH  - Prostheses and Implants
MH  - Tissue Inhibitor of Metalloproteinases/genetics/metabolism
PMC - PMC4940096
EDAT- 2016/07/01 06:00
MHDA- 2017/04/07 06:00
PMCR- 2016/06/21
CRDT- 2016/07/01 06:00
PHST- 2015/02/23 00:00 [received]
PHST- 2016/05/23 00:00 [accepted]
PHST- 2016/07/01 06:00 [entrez]
PHST- 2016/07/01 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
PHST- 2016/06/21 00:00 [pmc-release]
AID - mmr-14-02-1491 [pii]
AID - 10.3892/mmr.2016.5415 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Aug;14(2):1491-500. doi: 10.3892/mmr.2016.5415. Epub 2016 Jun 
      21.