PMID- 27357860
OWN - NLM
STAT- MEDLINE
DCOM- 20170412
LR  - 20190111
IS  - 1944-446X (Electronic)
IS  - 1000-467X (Print)
IS  - 1944-446X (Linking)
VI  - 35
IP  - 1
DP  - 2016 Jun 29
TI  - Protein stability regulators screening assay (Pro-SRSA): protein degradation 
      meets the CRISPR-Cas9 library.
PG  - 60
LID - 10.1186/s40880-016-0125-z [doi]
LID - 60
AB  - The regulation of protein stability is a fundamental issue for biophysical 
      processes, but there has not previously been a convenient and unbiased method of 
      identifying regulators of protein stability. However, as reported in the article 
      entitled "A genome-scale CRISPR-Cas9 screening method for protein stability 
      reveals novel regulators of Cdc25A," recently published in Cell Discovery, our 
      team developed a protein stability regulators screening assay (Pro-SRSA) by 
      combining the whole-genome clustered regularly interspaced short palindromic 
      repeats Cas9 (CRISPR-Cas9) library with a dual-fluorescence-based protein 
      stability reporter and high-throughput sequencing to screen for regulators of 
      protein stability. Based on our findings, we are confident that this efficient 
      and unbiased screening method at the genome scale will be used by researchers 
      worldwide to identify regulators of protein stability.
FAU - Wu, Yuanzhong
AU  - Wu Y
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road 
      East, Guangzhou, 510060, Guangdong, P. R. China.
FAU - Kang, Tiebang
AU  - Kang T
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road 
      East, Guangzhou, 510060, Guangdong, P. R. China. kangtb@sysucc.org.cn.
LA  - eng
PT  - Comment
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160629
PL  - England
TA  - Chin J Cancer
JT  - Chinese journal of cancer
JID - 101498232
SB  - IM
CON - Cell Discov. 2016 May 24;2:16014. PMID: 27462461
MH  - *Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Gene Library
MH  - Genome
MH  - Protein Stability
MH  - *Proteolysis
PMC - PMC4928262
OTO - NOTNLM
OT  - Acetylation
OT  - CRISPR-Cas9 screening
OT  - Cdc25A
OT  - Protein stability
OT  - Ubiquitination
EDAT- 2016/07/01 06:00
MHDA- 2017/04/13 06:00
PMCR- 2016/06/29
CRDT- 2016/07/01 06:00
PHST- 2016/06/06 00:00 [received]
PHST- 2016/06/13 00:00 [accepted]
PHST- 2016/07/01 06:00 [entrez]
PHST- 2016/07/01 06:00 [pubmed]
PHST- 2017/04/13 06:00 [medline]
PHST- 2016/06/29 00:00 [pmc-release]
AID - 10.1186/s40880-016-0125-z [pii]
AID - 125 [pii]
AID - 10.1186/s40880-016-0125-z [doi]
PST - epublish
SO  - Chin J Cancer. 2016 Jun 29;35(1):60. doi: 10.1186/s40880-016-0125-z.