PMID- 27358069
OWN - NLM
STAT- MEDLINE
DCOM- 20180503
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 30
TI  - Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization 
      dynamics through its action on F-actin organization in Sertoli cells.
PG  - 28589
LID - 10.1038/srep28589 [doi]
LID - 28589
AB  - Crumbs homolog 3 (or Crumbs3, CRB3) is a polarity protein expressed by Sertoli 
      and germ cells at the basal compartment in the seminiferous epithelium. CRB3 also 
      expressed at the blood-testis barrier (BTB), co-localized with F-actin, TJ 
      proteins occludin/ZO-1 and basal ES (ectoplasmic specialization) proteins 
      N-cadherin/beta-catenin at stages IV-VII only. The binding partners of CRB3 in the 
      testis were the branched actin polymerization protein Arp3, and the barbed 
      end-capping and bundling protein Eps8, illustrating its possible role in actin 
      organization. CRB3 knockdown (KD) by RNAi in Sertoli cells with an established 
      tight junction (TJ)-permeability barrier perturbed the TJ-barrier via changes in 
      the distribution of TJ- and basal ES-proteins at the cell-cell interface. These 
      changes were the result of CRB3 KD-induced re-organization of actin 
      microfilaments, in which actin microfilaments were truncated, and extensively 
      branched, thereby destabilizing F-actin-based adhesion protein complexes at the 
      BTB. Using Polyplus in vivo-jetPEI as a transfection medium with high efficiency 
      for CRB3 KD in the testis, the CRB3 KD testes displayed defects in spermatid and 
      phagosome transport, and also spermatid polarity due to a disruption of F-actin 
      organization. In summary, CRB3 is an actin microfilament regulator, playing a 
      pivotal role in organizing actin filament bundles at the ES.
FAU - Gao, Ying
AU  - Gao Y
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for 
      Biomedical Research, Population Council, 1230 York Ave, New York, 10065, New 
      York, USA.
FAU - Lui, Wing-Yee
AU  - Lui WY
AD  - School of Biological Sciences, University of Hong Kong, Hong Kong, China.
FAU - Lee, Will M
AU  - Lee WM
AD  - School of Biological Sciences, University of Hong Kong, Hong Kong, China.
FAU - Cheng, C Yan
AU  - Cheng CY
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for 
      Biomedical Research, Population Council, 1230 York Ave, New York, 10065, New 
      York, USA.
LA  - eng
GR  - R01 HD056034/HD/NICHD NIH HHS/United States
GR  - U54 HD029990/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160630
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Actin-Related Protein 3)
RN  - 0 (Actins)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Eps8 protein, rat)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Actin Cytoskeleton/*metabolism
MH  - Actin-Related Protein 3/metabolism
MH  - Actins/*metabolism
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Animals
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Sertoli Cells/cytology/*metabolism
MH  - Tight Junctions/*metabolism
PMC - PMC4928075
EDAT- 2016/07/01 06:00
MHDA- 2018/05/04 06:00
PMCR- 2016/06/30
CRDT- 2016/07/01 06:00
PHST- 2016/01/13 00:00 [received]
PHST- 2016/06/07 00:00 [accepted]
PHST- 2016/07/01 06:00 [entrez]
PHST- 2016/07/01 06:00 [pubmed]
PHST- 2018/05/04 06:00 [medline]
PHST- 2016/06/30 00:00 [pmc-release]
AID - srep28589 [pii]
AID - 10.1038/srep28589 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 30;6:28589. doi: 10.1038/srep28589.