PMID- 27364410
OWN - NLM
STAT- MEDLINE
DCOM- 20170907
LR  - 20180706
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 89
IP  - 2
DP  - 2017 Feb
TI  - Virus reactivations after autologous hematopoietic stem cell transplantation 
      detected by multiplex PCR assay.
PG  - 358-362
LID - 10.1002/jmv.24621 [doi]
AB  - Several studies have indicated that viral reactivations following allogeneic 
      hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral 
      reactivations after autologous HSCT (auto-HSCT) have not been investigated in 
      detail. We performed multiplex polymerase chain reaction (PCR) assay to examine 
      multiple viral reactivations simultaneously in 24 patients undergoing auto-HSCT 
      between September 2010 and December 2012. Weekly whole blood samples were 
      collected from pre- to 42 days post-HSCT, and tested for the following 13 
      viruses; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), 
      Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6), 
      HHV-7, HHV-8, adeno virus (ADV), BK virus (BKV), JC virus (JCV), parvovirus B19 
      (B19V), and hepatitis B virus (HBV).  Fifteen (63%) patients had at least one 
      type of viral reactivation. HHV6 (n = 10; 41.7%) was most frequently detected 
      followed by EBV (n = 7; 29.2%). HHV-6 peaked on day 21 after HSCT and promptly 
      declined. In addition, HBV, CMV, HHV7, and B19V were each detected in one 
      patient. HHV6 reactivation was detected in almost half the auto-HSCT patients, 
      which was similar to the incidence in allo-HSCT patients. The incidence of EBV 
      was unexpectedly high. Viral infections in patients undergoing auto-HSCT were 
      higher than previously reported in other studies. Although there were no 
      particular complications of viral infection, we should pay attention to possible 
      viral reactivations in auto-HSCT patients. J. Med. Virol. 89:358-362, 2017. (c) 
      2016 Wiley Periodicals, Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Inazawa, Natsuko
AU  - Inazawa N
AD  - Department of Pediatrics, JCHO Sapporo Hokushin Hospital, Sapporo, Japan.
FAU - Hori, Tsukasa
AU  - Hori T
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Nojima, Masanori
AU  - Nojima M
AD  - The Institute of Medical Science Hospital, Tokyo University, Tokyo, Japan.
FAU - Saito, Makoto
AU  - Saito M
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Igarashi, Keita
AU  - Igarashi K
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Yamamoto, Masaki
AU  - Yamamoto M
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Shimizu, Norio
AU  - Shimizu N
AD  - Department of Virology Medical Research Institute, Tokyo Medical and Dental 
      University, Tokyo, Japan.
FAU - Yoto, Yuko
AU  - Yoto Y
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Tsutsumi, Hiroyuki
AU  - Tsutsumi H
AD  - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20160727
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - DNA Viruses/classification/*isolation & purification
MH  - Female
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Multiplex Polymerase Chain Reaction
MH  - Prevalence
MH  - Prospective Studies
MH  - Transplantation, Autologous/*adverse effects
MH  - *Virus Activation
MH  - Young Adult
OTO - NOTNLM
OT  - autologous hematopoietic stem cell transplantation
OT  - multiplex PCR
OT  - virus infection
EDAT- 2016/07/02 06:00
MHDA- 2017/09/08 06:00
CRDT- 2016/07/02 06:00
PHST- 2016/06/26 00:00 [accepted]
PHST- 2016/07/02 06:00 [pubmed]
PHST- 2017/09/08 06:00 [medline]
PHST- 2016/07/02 06:00 [entrez]
AID - 10.1002/jmv.24621 [doi]
PST - ppublish
SO  - J Med Virol. 2017 Feb;89(2):358-362. doi: 10.1002/jmv.24621. Epub 2016 Jul 27.