PMID- 27372427 OWN - NLM STAT- MEDLINE DCOM- 20170523 LR - 20191210 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 477 IP - 4 DP - 2016 Sep 2 TI - Arabidopsis COP1-interacting protein 1 is a positive regulator of ABA response. PG - 847-853 LID - S0006-291X(16)31101-9 [pii] LID - 10.1016/j.bbrc.2016.06.147 [doi] AB - COP1-interacting protein 1 (CIP1, At5g41790) was the first reported interacting protein for CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) of Arabidopsis; however its physiological function has remained unknown for two decades. Here we show that CIP1 is a positive regulator of abscisic acid (ABA) response. CIP1 is mainly expressed in the photosynthetic cells and the vascular tissue, and its promoter activity can be induced by osmotic stress and ABA. The CIP1 protein is localized to the plasma membrane. A T-DNA insertion mutant cip1-1 was then characterized. The mutant is sensitive to osmotic stress and has ABA insensitive phenotypes. RNA sequencing showed that cip1-1 has lower levels of gene expression in abiotic stress response compared with the wild-type. Meanwhile, transcript levels of ABA biosynthesis genes are higher in cip1-1 than in the wild-type. These results suggested that CIP1 is positively involved in ABA response. CI - Copyright (c) 2016 Elsevier Inc. All rights reserved. FAU - Ren, Chenxia AU - Ren C AD - Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 94 Weijin Road, Nankai District, Tianjin 300071, PR China. Electronic address: rcx2010@126.com. FAU - Zhu, Xili AU - Zhu X AD - Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 94 Weijin Road, Nankai District, Tianjin 300071, PR China. Electronic address: zxlstc@163.com. FAU - Zhang, Pingping AU - Zhang P AD - Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 94 Weijin Road, Nankai District, Tianjin 300071, PR China. Electronic address: zhangppsmile@163.com. FAU - Gong, Qingqiu AU - Gong Q AD - Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 94 Weijin Road, Nankai District, Tianjin 300071, PR China. Electronic address: gongq@nankai.edu.cn. LA - eng PT - Journal Article DEP - 20160629 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Arabidopsis Proteins) RN - 72S9A8J5GW (Abscisic Acid) RN - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) SB - IM MH - Abscisic Acid/*administration & dosage MH - Arabidopsis/*physiology MH - Arabidopsis Proteins/*metabolism MH - Gene Expression Regulation, Plant/drug effects/*physiology MH - Osmotic Pressure/drug effects/physiology MH - Photosynthesis/drug effects/*physiology MH - Stress, Physiological/*physiology MH - Tissue Distribution MH - Ubiquitin-Protein Ligases OTO - NOTNLM OT - Abscisic acid OT - COP1-interacting protein 1 OT - Osmotic stress OT - RNA sequencing OT - Sub-cellular localization EDAT- 2016/07/04 06:00 MHDA- 2017/05/24 06:00 CRDT- 2016/07/04 06:00 PHST- 2016/06/27 00:00 [received] PHST- 2016/06/28 00:00 [accepted] PHST- 2016/07/04 06:00 [entrez] PHST- 2016/07/04 06:00 [pubmed] PHST- 2017/05/24 06:00 [medline] AID - S0006-291X(16)31101-9 [pii] AID - 10.1016/j.bbrc.2016.06.147 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2016 Sep 2;477(4):847-853. doi: 10.1016/j.bbrc.2016.06.147. Epub 2016 Jun 29.