PMID- 27372913
OWN - NLM
STAT- MEDLINE
DCOM- 20170308
LR  - 20170308
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1043
DP  - 2017 Feb 1
TI  - Facial synthesis of nickel(II)-immobilized carboxyl cotton chelator for 
      purification of histidine-tagged proteins.
PG  - 122-127
LID - S1570-0232(16)30418-4 [pii]
LID - 10.1016/j.jchromb.2016.06.029 [doi]
AB  - Immobilized metal affinity chromatography (IMAC) technique is frequently used in 
      the purification of histidine-tagged (His-tagged) recombinant proteins. In this 
      study, nickel(II)-immobilized carboxyl cotton chelator (CCC-Ni(2+)) fibers was 
      synthesized by a simple method based on the coordination effect between Ni(2+) 
      and carboxyl group. The nickel content of the CCC-Ni(2+) fibers was determined to 
      be 5 times larger than that of Ni(2+)-immobilized sulfhydryl cotton fiber 
      (SCF-Ni(2+)) fibers developed in our previous work. The prepared CCC-Ni(2+) 
      fibers were then applied for the selective and rapid separation of His-tagged 
      protein from escherichia coli (E. coli) cell lysates on the basis of the high 
      affinity of Ni(2+) to 6xHis with a lab-in-syringe format. Benefiting from the 
      good biological compatibility and high nickel content, the results showed that 
      CCC-Ni(2+) fibers were able to selectively capture His-tagged proteins from 
      complex E. coli cell lysates and exhibited a relatively large adsorption capacity 
      toward His-tagged protein. The recoveries of His-tagged GFP in E. coli cell 
      lysates were in the range of 89.8%-106.7% with the relative standard deviations 
      (RSDs) less than 9.4% (intra-day) and 10.3% (inter-day). Taken together, this 
      efficient approach for the purification of recombinant proteins extends the 
      application of CCC-based fibrous materials in biological analysis.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - He, Xiao-Mei
AU  - He XM
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160618
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Chelating Agents)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 4QD397987E (Histidine)
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Chelating Agents/chemistry
MH  - Chromatography, Affinity/*instrumentation/*methods
MH  - Cotton Fiber
MH  - Histidine/*chemistry
MH  - Nickel/*chemistry
MH  - Recombinant Fusion Proteins/analysis/chemistry/*isolation & purification
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - E. coli cell lysates
OT  - His-tagged
OT  - Immobilized metal ion affinity chromatography (IMAC)
OT  - Ni(2+)-immobilized carboxyl cotton chelator (CCC-Ni(2+))
OT  - Proteins
EDAT- 2016/07/04 06:00
MHDA- 2017/03/09 06:00
CRDT- 2016/07/04 06:00
PHST- 2016/04/06 00:00 [received]
PHST- 2016/06/08 00:00 [revised]
PHST- 2016/06/17 00:00 [accepted]
PHST- 2016/07/04 06:00 [pubmed]
PHST- 2017/03/09 06:00 [medline]
PHST- 2016/07/04 06:00 [entrez]
AID - S1570-0232(16)30418-4 [pii]
AID - 10.1016/j.jchromb.2016.06.029 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Feb 1;1043:122-127. doi: 
      10.1016/j.jchromb.2016.06.029. Epub 2016 Jun 18.