PMID- 27373675
OWN - NLM
STAT- MEDLINE
DCOM- 20170308
LR  - 20190221
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 36
IP  - 2
DP  - 2016 Aug
TI  - Activation of the FAK/PI3K pathway is crucial for AURKA-induced 
      epithelial-mesenchymal transition in laryngeal cancer.
PG  - 819-26
LID - 10.3892/or.2016.4872 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant 
      tumors, and the main cause of death is metastasis. Overexpression of aurora 
      kinase A (AURKA) plays an important role in the metastasis of LSCC. However, the 
      mechanism by which AURKA promotes the metastasis of LSCC is poorly understood. 
      Recent accumulating evidence indicates that epithelial-mesenchymal transition 
      (EMT) may be one of the mechanisms of tumor metastasis. In the present study, we 
      studied whether AURKA may induce EMT to promote the metastasis of LSCC. CCK-8 and 
      plate colony-formation assays were carried out to show that AURKA significantly 
      promoted the proliferation of Hep2 cells. Immunofluorescence staining and western 
      blotting showed that EMT-related proteins changed in a time-dependent manner 
      along with the alteration of AURKA, with decreased expression of N-cadherin, 
      vimentin and slug and increased expression of E-cadherin. Additionally, 
      downregulation of the expression of AURKA inhibited FAK/PI3K pathway activity. 
      Inhibition of the FAK/PI3K pathway caused less mesenchymal-like characteristics 
      and reduced the mobility, migration and invasion of Hep2 cells. In conclusion, 
      AURKA may induce EMT to promote metastasis via activation of the FAK/PI3K pathway 
      in LSCC. Those regulatory factors may present new diagnostic biomarkers and 
      potential therapeutic targets for LSCC.
FAU - Yang, Liyun
AU  - Yang L
AD  - Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200025, P.R. China.
FAU - Zhou, Quan
AU  - Zhou Q
AD  - Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive 
      Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 
      Shanghai 200025, P.R. China.
FAU - Chen, Xuehua
AU  - Chen X
AD  - Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive 
      Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 
      Shanghai 200025, P.R. China.
FAU - Su, Liping
AU  - Su L
AD  - Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive 
      Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 
      Shanghai 200025, P.R. China.
FAU - Liu, Bingya
AU  - Liu B
AD  - Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive 
      Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 
      Shanghai 200025, P.R. China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai 200025, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160613
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Cadherins)
RN  - 0 (SNAI1 protein, human)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (Vimentin)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (PTK2 protein, human)
RN  - EC 2.7.11.1 (AURKA protein, human)
RN  - EC 2.7.11.1 (Aurora Kinase A)
RN  - M03GIQ7Z6P (Sincalide)
SB  - IM
MH  - Aurora Kinase A/*genetics
MH  - Cadherins/genetics
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/genetics
MH  - Epithelial-Mesenchymal Transition/*genetics
MH  - Focal Adhesion Kinase 1/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Head and Neck Neoplasms/genetics/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis/genetics/pathology
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Phosphatidylinositol 3-Kinases/*genetics
MH  - Signal Transduction/genetics
MH  - Sincalide/genetics
MH  - Snail Family Transcription Factors/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Vimentin/genetics
EDAT- 2016/07/05 06:00
MHDA- 2017/03/09 06:00
CRDT- 2016/07/05 06:00
PHST- 2016/01/25 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/07/05 06:00 [entrez]
PHST- 2016/07/05 06:00 [pubmed]
PHST- 2017/03/09 06:00 [medline]
AID - 10.3892/or.2016.4872 [doi]
PST - ppublish
SO  - Oncol Rep. 2016 Aug;36(2):819-26. doi: 10.3892/or.2016.4872. Epub 2016 Jun 13.