PMID- 27374910
OWN - NLM
STAT- MEDLINE
DCOM- 20170210
LR  - 20170210
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 191
DP  - 2016 Aug 15
TI  - Comparison of protection provided by type 1 and type 2 porcine reproductive and 
      respiratory syndrome field viruses against homologous and heterologous challenge.
PG  - 72-81
LID - S0378-1135(16)30158-4 [pii]
LID - 10.1016/j.vetmic.2016.06.003 [doi]
AB  - The objective of this study was to compare protection provided by type 1 and type 
      2 porcine reproductive and respiratory syndrome virus (PRRSV) against homologous 
      and heterologous challenge based on clinical, virological, immunological, and 
      pathological analysis. At 3 and 8 weeks of age, pigs were inoculated intranasally 
      with either 3mL of tissue culture fluid containing 10(5) TCID50/mL of type 1 
      PRRSV or 3mL of tissue culture fluid containing 10(5) TCID50/mL of type 2 PRRSV. 
      The homologous challenges resulted in a significant boost of the neutralizing 
      antibodies (NA) and interferon-gamma secreting cells (IFN-gamma-SC) compared to 
      heterologous challenges. The reduction of secondary challenging PRRSV viremia 
      coincided with the appearance of homologous PRRSV-specific NA and IFN-gamma-SC. 
      Homologous challenge reduced the severity of lung lesions and levels of PRRSV 
      viremia significantly in pigs in comparison with heterologous challenge. The 
      differences in homologous and heterologous NA and IFN-gamma-SC response may explain 
      the differences in protection against homologous and heterologous challenge 
      between type 1 and type 2 PRRSV. Primary challenge (immunization) with type 1 
      PRRSV provided protection against the secondary homologous challenge with type 1 
      PRRSV but failed to provide protection against the secondary heterologous 
      challenge of type 2 PRRSV. Primary challenge with type 2 PRRSV provided 
      protection against both the secondary homologous challenge with type 2 PRRSV and 
      the secondary heterologous challenge with type 1 PRRSV.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Choi, Kyuhyung
AU  - Choi K
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Park, Changhoon
AU  - Park C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Jeong, Jiwoon
AU  - Jeong J
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Chae, Chanhee
AU  - Chae C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea. 
      Electronic address: swine@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160607
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood/immunology
MH  - Cross Protection/*immunology
MH  - Immunization/standards/*veterinary
MH  - Lung/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Random Allocation
MH  - Swine
OTO - NOTNLM
OT  - Genotype
OT  - Heterologous challenge
OT  - Homologous challenge
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2016/07/05 06:00
MHDA- 2017/02/12 06:00
CRDT- 2016/07/05 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/06/01 00:00 [revised]
PHST- 2016/06/06 00:00 [accepted]
PHST- 2016/07/05 06:00 [entrez]
PHST- 2016/07/05 06:00 [pubmed]
PHST- 2017/02/12 06:00 [medline]
AID - S0378-1135(16)30158-4 [pii]
AID - 10.1016/j.vetmic.2016.06.003 [doi]
PST - ppublish
SO  - Vet Microbiol. 2016 Aug 15;191:72-81. doi: 10.1016/j.vetmic.2016.06.003. Epub 
      2016 Jun 7.