PMID- 27375428
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160704
LR  - 20200930
IS  - 1662-5099 (Print)
IS  - 1662-5099 (Electronic)
IS  - 1662-5099 (Linking)
VI  - 9
DP  - 2016
TI  - The Enigma of the Dichotomic Pressure Response of GluN1-4a/b Splice Variants of 
      NMDA Receptor: Experimental and Statistical Analyses.
PG  - 40
LID - 10.3389/fnmol.2016.00040 [doi]
LID - 40
AB  - Professional deep-water divers, exposed to hyperbaric pressure (HP) above 1.1 
      MPa, develop High Pressure Neurological Syndrome (HPNS), which is associated with 
      central nervous system (CNS) hyperexcitability. It was previously reported that 
      HP augments N-methyl-D-aspartate receptor (NMDAR) synaptic response, increases 
      neuronal excitability and potentially causes irreversible neuronal damage. Our 
      laboratory has reported differential current responses under HP conditions in 
      NMDAR subtypes that contain either GluN1-1a or GluN1-1b splice variants 
      co-expressed in Xenopus laevis oocytes with all four GluN2 subunits. Recently, we 
      reported that the increase in ionic currents measured under HP conditions is also 
      dependent on which of the eight splice variants of GluN1 is co-expressed with the 
      GluN2 subunit. We now report that the NMDAR subtype that contains GluN1-4a/b 
      splice variants exhibited "dichotomic" (either increased or decreased) responses 
      at HP. The distribution of the results is not normal thus analysis of variance 
      (ANOVA) test and clustering analysis were employed for statistical verification 
      of the grouping. Furthermore, the calculated constants of alpha function 
      distribution analysis for the two groups were similar, suggesting that the 
      mechanism underlying the switch between an increase or a decrease of the current 
      at HP is a single process, the nature of which is still unknown. This dichotomic 
      response of the GluN1-4a/b splice variant may be used as a model for studying 
      reduced response in NMDAR at HP. Successful reversal of other NMDAR subtypes 
      response (i.e., current reduction) may allow the elimination of the reversible 
      malfunctioning short term effects (HPNS), or even deleterious long term effects 
      induced by increased NMDAR function during HP exposure.
FAU - Bliznyuk, Alice
AU  - Bliznyuk A
AD  - Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, 
      Ben-Gurion University of the Negev Beer-sheva, Israel.
FAU - Gradwohl, Gideon
AU  - Gradwohl G
AD  - Department of Physics, Jerusalem College of Technology Jerusalem, Israel.
FAU - Hollmann, Michael
AU  - Hollmann M
AD  - Department of Biochemistry I-Receptor Biochemistry, Ruhr University Bochum 
      Bochum, Germany.
FAU - Grossman, Yoram
AU  - Grossman Y
AD  - Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, 
      Ben-Gurion University of the Negev Beer-sheva, Israel.
LA  - eng
PT  - Journal Article
DEP - 20160610
PL  - Switzerland
TA  - Front Mol Neurosci
JT  - Frontiers in molecular neuroscience
JID - 101477914
PMC - PMC4901057
OTO - NOTNLM
OT  - CNS hyperexcitability
OT  - HPNS
OT  - diving physiology
OT  - oocyte
OT  - pressure
EDAT- 2016/07/05 06:00
MHDA- 2016/07/05 06:01
PMCR- 2016/01/01
CRDT- 2016/07/05 06:00
PHST- 2016/01/25 00:00 [received]
PHST- 2016/05/17 00:00 [accepted]
PHST- 2016/07/05 06:00 [entrez]
PHST- 2016/07/05 06:00 [pubmed]
PHST- 2016/07/05 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fnmol.2016.00040 [doi]
PST - epublish
SO  - Front Mol Neurosci. 2016 Jun 10;9:40. doi: 10.3389/fnmol.2016.00040. eCollection 
      2016.