PMID- 27376399
OWN - NLM
STAT- MEDLINE
DCOM- 20170906
LR  - 20171204
IS  - 1365-2583 (Electronic)
IS  - 0962-1075 (Linking)
VI  - 25
IP  - 6
DP  - 2016 Dec
TI  - Cotesia vestalis parasitization suppresses expression of a Plutella xylostella 
      thioredoxin.
PG  - 679-688
LID - 10.1111/imb.12252 [doi]
AB  - Thioredoxins (Trxs) are a family of small, highly conserved and ubiquitous 
      proteins involved in protecting organisms against toxic reactive oxygen species. 
      In this study, a typical thioredoxin gene, PxTrx, was isolated from Plutella 
      xylostella. The full-length cDNA sequence is composed of 959 bp containing a 321 
      bp open reading frame that encodes a predicted protein of 106 amino acids, a 
      predicted molecular weight of 11.7 kDa and an isoelectric point of 5.03. PxTrx 
      was mainly expressed in larval Malpighian tubules and the fat body. An enriched 
      recombinant PxTrx had insulin disulphide reductase activity and stimulated Human 
      Embryonic Kidney 293 (HEK293) cell proliferation. It also protected supercoiled 
      DNA and living HEK293 cells from H(2) O(2) -induced damage. Parasitization by 
      Cotesia vestalis and injections of 0.05 and 0.01 equivalents of C. vestalis 
      Bracovirus (CvBv), the symbiotic virus carried by the parasitoid, led to 
      down-regulation of PxTrx expression in host fat body. Taken together, our results 
      indicate that PxTrx contributes to the maintenance of P. xylostella cellular 
      haemostasis. Host fat body expression of PxTrx is strongly attenuated by 
      parasitization and by injections of CvBv.
CI  - (c) 2016 The Royal Entomological Society.
FAU - Shi, M
AU  - Shi M
AD  - Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect 
      Sciences, Zhejiang University, Hangzhou, China.
FAU - Zhao, S
AU  - Zhao S
AD  - Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect 
      Sciences, Zhejiang University, Hangzhou, China.
FAU - Wang, Z-H
AU  - Wang ZH
AD  - Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect 
      Sciences, Zhejiang University, Hangzhou, China.
FAU - Stanley, D
AU  - Stanley D
AD  - Biological Control of Insects Research Laboratory, Agricultural Research Service, 
      U.S. Department of Agriculture, Columbia, MO, USA.
FAU - Chen, X-X
AU  - Chen XX
AD  - Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect 
      Sciences, Zhejiang University, Hangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160704
PL  - England
TA  - Insect Mol Biol
JT  - Insect molecular biology
JID - 9303579
RN  - 0 (DNA, Complementary)
RN  - 0 (Insect Proteins)
RN  - 0 (RNA, Messenger)
RN  - 52500-60-4 (Thioredoxins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/metabolism
MH  - Down-Regulation/*physiology
MH  - Fat Body/metabolism
MH  - Female
MH  - *Host-Parasite Interactions
MH  - Insect Proteins/chemistry/*genetics/metabolism
MH  - Larva/genetics/parasitology/physiology
MH  - Male
MH  - Moths/*genetics/metabolism/*parasitology
MH  - Pupa/genetics/parasitology/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Thioredoxins/chemistry/*genetics/metabolism
MH  - Wasps/*physiology
OTO - NOTNLM
OT  - Plutella xylostella
OT  - antioxidant capacity
OT  - expression profile
OT  - parasitization
OT  - thioredoxin
EDAT- 2016/07/05 06:00
MHDA- 2017/09/07 06:00
CRDT- 2016/07/05 06:00
PHST- 2016/07/05 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2016/07/05 06:00 [entrez]
AID - 10.1111/imb.12252 [doi]
PST - ppublish
SO  - Insect Mol Biol. 2016 Dec;25(6):679-688. doi: 10.1111/imb.12252. Epub 2016 Jul 4.