PMID- 27378424
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20171126
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 477
IP  - 4
DP  - 2016 Sep 2
TI  - Open reading frames 1a and 1b of the porcine reproductive and respiratory 
      syndrome virus (PRRSV) collaboratively initiate viral minus-strand RNA synthesis.
PG  - 927-931
LID - S0006-291X(16)31115-9 [pii]
LID - 10.1016/j.bbrc.2016.06.161 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) causes a 
      persistent threat to the swine industry, especially when highly pathogenic PRRSV 
      (HP-PRRSV) emerges. Previous studies have indicated that PRRSV RNA synthesis was 
      correlated with HP-PRRSV virulence. PRRSV RNA synthesis includes genomic RNA and 
      sub-genomic mRNA, and these processes require minus-strand RNA as a template. 
      However, the mechanisms involved in PRRSV minus-strand RNA synthesis are not 
      fully understood. A mini-genome system can be used to assess viral replication 
      mechanisms and to evaluate the effects of potential antiviral drugs on viral 
      replicase activities. In this study, we developed a mini-genome system that uses 
      firefly luciferase as a reporter. Based on this system, we found that PRRSV 
      RNA-dependent RNA polymerase nsp9 alone failed to activate virus minus-strand RNA 
      synthesis. We also demonstrated that combinations of open reading frames 1a 
      (ORF1a) and ORF1b are necessary for viral minus-strand RNA synthesis.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Tang, Yan-Dong
AU  - Tang YD
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Fang, Qiong-Qiong
AU  - Fang QQ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Liu, Ji-Ting
AU  - Liu JT
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China; 
      College of Animal Science and Technology, Jilin Agriculture University, Changchun 
      130018, China.
FAU - Wang, Tong-Yun
AU  - Wang TY
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Tao, Ye
AU  - Tao Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Liu, Yong-Gang
AU  - Liu YG
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China. 
      Electronic address: yongangliu@aliyun.com.
FAU - Cai, Xue-Hui
AU  - Cai XH
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China. 
      Electronic address: cai139@hvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160701
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping/methods
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA, Viral/*genetics
MH  - Swine
MH  - Transcription Elongation, Genetic/*physiology
MH  - Virus Activation/*genetics
OTO - NOTNLM
OT  - Mini-genome system
OT  - PRRSV
OT  - RNA synthesis
EDAT- 2016/07/06 06:00
MHDA- 2017/05/16 06:00
CRDT- 2016/07/06 06:00
PHST- 2016/06/28 00:00 [received]
PHST- 2016/06/30 00:00 [accepted]
PHST- 2016/07/06 06:00 [entrez]
PHST- 2016/07/06 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
AID - S0006-291X(16)31115-9 [pii]
AID - 10.1016/j.bbrc.2016.06.161 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Sep 2;477(4):927-931. doi: 
      10.1016/j.bbrc.2016.06.161. Epub 2016 Jul 1.