PMID- 27379407
OWN - NLM
STAT- MEDLINE
DCOM- 20170217
LR  - 20170811
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1028
DP  - 2016 Aug 15
TI  - Simultaneous quantification of monoamine neurotransmitters and their biogenic 
      metabolites intracellularly and extracellularly in primary neuronal cell cultures 
      and in sub-regions of guinea pig brain.
PG  - 222-230
LID - S1570-0232(16)30361-0 [pii]
LID - 10.1016/j.jchromb.2016.05.048 [doi]
AB  - In the present paper, we describe a validated chromatographic method for the 
      simultaneous quantification of monoamine neurotransmitters and their biogenic 
      metabolites intracellularly and extracellularly in primary neuronal cell culture 
      and in sub-regions of the guinea pig brain. Electrochemical detection provided 
      limits of quantifications (LOQs) between 3.6 and 12nM. Within the linear range, 
      obtained recoveries were from 90.9+/-9.9 to 120+/-14% and intra-day and inter-day 
      precisions found to be less than 5.5% and 12%, respectively. The analytical 
      method was applicable for quantification of intracellular and extracellular 
      amounts of monoamine neurotransmitters and their metabolites in guinea pig 
      frontal cortex and hippocampal primary neuronal cell cultures. Noradrenaline, 
      dopamine and serotonin were found to be in a range from 0.31 to 1.7pmol per 2 
      million cells intracellularly, but only the biogenic metabolites could be 
      detected extracellularly. Distinct differences in monoamine concentrations were 
      observed when comparing concentrations in guinea pig frontal cortex and 
      cerebellum tissue with higher amounts of dopamine and its metabolites, 
      3,4-dihydroxyphenylacetic acid and homovanillic acid in frontal cortex, as 
      compared to cerebellum. The chemical turnover in frontal cortex tissue of guinea 
      pig was for serotonin successfully predicted from the turnover observed in the 
      frontal cortex cell culture. In conclusion, the present analytical method shows 
      high precision, accuracy and sensitivity and is broadly applicable to monoamine 
      measurements in cell cultures as well as brain biopsies from animal models used 
      in preclinical neurochemistry.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Schou-Pedersen, Anne Marie V
AU  - Schou-Pedersen AMV
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg C, Denmark. 
      Electronic address: am.schoupedersen@sund.ku.dk.
FAU - Hansen, Stine N
AU  - Hansen SN
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg C, Denmark.
FAU - Tveden-Nyborg, Pernille
AU  - Tveden-Nyborg P
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg C, Denmark.
FAU - Lykkesfeldt, Jens
AU  - Lykkesfeldt J
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg C, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20160616
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Biogenic Monoamines)
RN  - 0 (Neurotransmitter Agents)
SB  - IM
MH  - Animals
MH  - Biogenic Monoamines/*analysis/metabolism
MH  - Brain/metabolism
MH  - *Brain Chemistry
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid/methods
MH  - Electrochemical Techniques/methods
MH  - Guinea Pigs
MH  - Neurons/*chemistry/metabolism
MH  - Neurotransmitter Agents/*analysis/metabolism
MH  - Primary Cell Culture
OTO - NOTNLM
OT  - Cell culture
OT  - Electrochemical detection
OT  - Guinea pig
OT  - HPLC
OT  - Monoamines
EDAT- 2016/07/06 06:00
MHDA- 2017/02/18 06:00
CRDT- 2016/07/06 06:00
PHST- 2016/04/28 00:00 [received]
PHST- 2016/05/27 00:00 [revised]
PHST- 2016/05/28 00:00 [accepted]
PHST- 2016/07/06 06:00 [entrez]
PHST- 2016/07/06 06:00 [pubmed]
PHST- 2017/02/18 06:00 [medline]
AID - S1570-0232(16)30361-0 [pii]
AID - 10.1016/j.jchromb.2016.05.048 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Aug 15;1028:222-230. doi: 
      10.1016/j.jchromb.2016.05.048. Epub 2016 Jun 16.