PMID- 27379522
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 7
DP  - 2016
TI  - Estradiol Exposure Differentially Alters Monolayer versus Microtissue MCF-7 Human 
      Breast Carcinoma Cultures.
PG  - e0157997
LID - 10.1371/journal.pone.0157997 [doi]
LID - e0157997
AB  - The development of three-dimensional (3D) cultures is increasing, as they are 
      able to provide the utility of in vitro models and the strength of testing in 
      physiologically relevant systems. When cultured in a scaffold-free agarose 
      hydrogel system, MCF-7 human breast carcinoma cells organize and develop into 
      microtissues that contain a luminal space, in stark contrast to the flat 
      morphology of MCF-7 two-dimensional (2D) monolayer cultures. Following exposure 
      to 1nM E2, expression of typical estrogen-responsive genes, including 
      progesterone receptor (PGR), PDZ containing domain 1 (PDZK1) and amphiregulin 
      (AREG) is increased in both 2D and 3D cultures. When examining expression of 
      other genes, particularly those involved in cell adhesion, there were large 
      changes in 3D MCF-7 microtissues, with little to no change observed in the MCF-7 
      monolayer cultures. Together, these results indicate that while the initial 
      estrogen-regulated transcriptional targets respond similarly in 2D and 3D 
      cultures, there are large differences in activation of other pathways related to 
      cell-cell interactions.
FAU - Vantangoli, Marguerite M
AU  - Vantangoli MM
AUID- ORCID: 0000-0001-8757-440X
AD  - Department of Pathology and Laboratory Medicine, Brown University, 70 Ship 
      Street, Providence RI 02903, United States of America.
FAU - Madnick, Samantha J
AU  - Madnick SJ
AD  - Department of Pathology and Laboratory Medicine, Brown University, 70 Ship 
      Street, Providence RI 02903, United States of America.
FAU - Wilson, Shelby
AU  - Wilson S
AD  - Department of Pathology and Laboratory Medicine, Brown University, 70 Ship 
      Street, Providence RI 02903, United States of America.
FAU - Boekelheide, Kim
AU  - Boekelheide K
AD  - Department of Pathology and Laboratory Medicine, Brown University, 70 Ship 
      Street, Providence RI 02903, United States of America.
LA  - eng
GR  - R01 ES020750/ES/NIEHS NIH HHS/United States
GR  - T32 ES007272/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20160705
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Estrogens)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 4TI98Z838E (Estradiol)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Adhesion/genetics
MH  - Cell Communication/genetics
MH  - Cell Culture Techniques/*methods
MH  - Cluster Analysis
MH  - Estradiol/*pharmacology
MH  - Estrogens/pharmacology
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/metabolism
MH  - MCF-7 Cells
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sepharose/metabolism
MH  - Signal Transduction/genetics
PMC - PMC4933361
COIS- Competing Interests: The authors have read the journal's policy and have the 
      following competing interests: Kim Boekelheide is an occasional expert consultant 
      for chemical and pharmaceutical companies, and owns stock in Semma Therapeutics, 
      a biotechnology company developing a cell-based therapy for diabetes. This does 
      not alter the authors' adherence to PLOS ONE policies on sharing data and 
      materials.
EDAT- 2016/07/06 06:00
MHDA- 2017/08/02 06:00
PMCR- 2016/07/05
CRDT- 2016/07/06 06:00
PHST- 2016/01/06 00:00 [received]
PHST- 2016/06/08 00:00 [accepted]
PHST- 2016/07/06 06:00 [entrez]
PHST- 2016/07/06 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2016/07/05 00:00 [pmc-release]
AID - PONE-D-16-00668 [pii]
AID - 10.1371/journal.pone.0157997 [doi]
PST - epublish
SO  - PLoS One. 2016 Jul 5;11(7):e0157997. doi: 10.1371/journal.pone.0157997. 
      eCollection 2016.