PMID- 27380936
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20190111
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jul 6
TI  - Telomere Visualization in Tissue Sections using Pyrrole-Imidazole Polyamide 
      Probes.
PG  - 29261
LID - 10.1038/srep29261 [doi]
LID - 29261
AB  - Pyrrole-Imidazole (PI) polyamides bind to specific DNA sequences in the minor 
      groove with high affinity. Specific DNA labeling by PI polyamides does not 
      require DNA denaturation with harsh treatments of heat and formamide and has the 
      advantages of rapid and less disruptive processing. Previously, we developed 
      tandem hairpin PI polyamide probes (TH59 series), which label telomeres in 
      cultured cell lines more efficiently than conventional methods, such as 
      fluorescence in situ hybridization (FISH). Here, we demonstrate that a TH59 
      derivative, HPTH59-b, along with immunostaining for specifying cell types in the 
      tissues, visualizes telomeres in mouse and human tissue sections. Quantitative 
      measurements of telomere length with single-cell resolution suggested shorter 
      telomeres in the proliferating cell fractions of tumor than in non-tumor tissues. 
      Thus, PI polyamides are a promising alternative for telomere labeling in clinical 
      research, as well as in cell biology.
FAU - Sasaki, Asuka
AU  - Sasaki A
AD  - Structural Biology Center, National Institute of Genetics, and Department of 
      Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 
      411-8540, Japan.
FAU - Ide, Satoru
AU  - Ide S
AD  - Structural Biology Center, National Institute of Genetics, and Department of 
      Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 
      411-8540, Japan.
FAU - Kawamoto, Yusuke
AU  - Kawamoto Y
AD  - Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, 
      Kyoto 606-8502, Japan.
FAU - Bando, Toshikazu
AU  - Bando T
AD  - Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, 
      Kyoto 606-8502, Japan.
FAU - Murata, Yukinori
AU  - Murata Y
AD  - Department of Pathology, Hospital, National Center for Global Health and 
      Medicine, Shinjuku, Tokyo 162-8655, Japan.
FAU - Shimura, Mari
AU  - Shimura M
AD  - Department of Intractable Diseases, Research Institute, National Center for 
      Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
FAU - Yamada, Kazuhiko
AU  - Yamada K
AD  - Department of Surgery, Hospital, National Center for Global Health and Medicine, 
      Shinjuku, Tokyo 162-8655, Japan.
FAU - Hirata, Akiyoshi
AU  - Hirata A
AD  - HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto 602-8158, Japan.
FAU - Nokihara, Kiyoshi
AU  - Nokihara K
AD  - HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto 602-8158, Japan.
FAU - Hirata, Tatsumi
AU  - Hirata T
AD  - Division of Brain Function, Department of Integrated Genetics, National Institute 
      of Genetics, and Department of Genetics, Sokendai, Mishima, Shizuoka 411-8540, 
      Japan.
FAU - Sugiyama, Hiroshi
AU  - Sugiyama H
AD  - Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, 
      Kyoto 606-8502, Japan.
FAU - Maeshima, Kazuhiro
AU  - Maeshima K
AD  - Structural Biology Center, National Institute of Genetics, and Department of 
      Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 
      411-8540, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160706
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Imidazoles)
RN  - 0 (Nylons)
RN  - 0 (Pyrroles)
RN  - 7GBN705NH1 (imidazole)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cytological Techniques/methods
MH  - DNA/*metabolism
MH  - Histocytochemistry/methods
MH  - Humans
MH  - Imidazoles/*metabolism
MH  - Mice
MH  - Nylons/*metabolism
MH  - Optical Imaging/*methods
MH  - Pyrroles/*metabolism
MH  - Staining and Labeling/*methods
MH  - Telomere/*metabolism
PMC - PMC4933941
EDAT- 2016/07/07 06:00
MHDA- 2018/05/23 06:00
PMCR- 2016/07/06
CRDT- 2016/07/07 06:00
PHST- 2016/05/12 00:00 [received]
PHST- 2016/06/13 00:00 [accepted]
PHST- 2016/07/07 06:00 [entrez]
PHST- 2016/07/07 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2016/07/06 00:00 [pmc-release]
AID - srep29261 [pii]
AID - 10.1038/srep29261 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jul 6;6:29261. doi: 10.1038/srep29261.