PMID- 27382057
OWN - NLM
STAT- MEDLINE
DCOM- 20170512
LR  - 20210205
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 291
IP  - 35
DP  - 2016 Aug 26
TI  - Chromatin Remodeler Recruitment during Macrophage Differentiation Facilitates 
      Transcription Factor Binding to Enhancers in Mature Cells.
PG  - 18058-18071
LID - 10.1074/jbc.M116.734186 [doi]
AB  - We show how enhancers of macrophage-specific genes are rendered accessible in 
      differentiating macrophages to allow their induction in mature cells in response 
      to an appropriate stimulus. Using a lentiviral knockdown approach in primary 
      differentiating macrophages from mouse bone marrow, we demonstrate that enhancers 
      of Il12b and Il1a are kept relatively lowly occupied by nucleosomes and 
      accessible through recruitment of the nucleosome remodeler BAF/PBAF. Our results 
      using an inducible cell line that expresses an estrogen receptor fusion of the 
      macrophage-specific transcription factor PU.1 (PUER) show that BAF/PBAF 
      recruitment to these enhancers is a consequence of translocation of PUER to the 
      nucleus in the presence of tamoxifen, and we speculate that remodeler recruitment 
      may be directly mediated by PU.1. In the absence of BAF/PBAF recruitment, 
      nucleosome occupancy at the enhancer of Il12b (and to a lesser extent at Il1a) 
      reaches high levels in bone marrow-derived macrophages (BMDMs), and the enhancers 
      are not fully cleared of nucleosomes upon LPS induction, resulting in impaired 
      gene expression. Analysis of Il12b expression in single cells suggests that 
      recruitment of the remodeler is necessary for high levels of transcription from 
      the same promoter, and we propose that remodelers function by increasing 
      nucleosome turnover to facilitate transcription factor over nucleosome binding in 
      a process we have termed "remodeler-assisted competition."
CI  - (c) 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - McAndrew, Michael J
AU  - McAndrew MJ
AD  - the Genetics Graduate Program, Michigan State University, East Lansing, Michigan 
      48824.
AD  - From the Department of Biochemistry and Molecular Biology and.
FAU - Gjidoda, Alison
AU  - Gjidoda A
AD  - From the Department of Biochemistry and Molecular Biology and.
FAU - Tagore, Mohita
AU  - Tagore M
AD  - the Genetics Graduate Program, Michigan State University, East Lansing, Michigan 
      48824.
AD  - From the Department of Biochemistry and Molecular Biology and.
FAU - Miksanek, Tyler
AU  - Miksanek T
AD  - From the Department of Biochemistry and Molecular Biology and.
FAU - Floer, Monique
AU  - Floer M
AUID- ORCID: 0000-0001-9593-9069
AD  - the Genetics Graduate Program, Michigan State University, East Lansing, Michigan 
      48824 floer@msu.edu.
AD  - From the Department of Biochemistry and Molecular Biology and.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160705
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Nucleosomes)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SWI-SNF-B chromatin-remodeling complex)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Chromosomal Proteins, Non-Histone/genetics/*metabolism
MH  - Enhancer Elements, Genetic/*physiology
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Nucleosomes/genetics/metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC5000056
OTO - NOTNLM
OT  - chromatin
OT  - chromatin remodeler
OT  - chromatin remodeling
OT  - gene expression
OT  - gene regulation
OT  - macrophage
OT  - nucleosome occupancy
EDAT- 2016/07/07 06:00
MHDA- 2017/05/13 06:00
PMCR- 2017/08/26
CRDT- 2016/07/07 06:00
PHST- 2016/04/21 00:00 [received]
PHST- 2016/07/07 06:00 [entrez]
PHST- 2016/07/07 06:00 [pubmed]
PHST- 2017/05/13 06:00 [medline]
PHST- 2017/08/26 00:00 [pmc-release]
AID - S0021-9258(20)32171-2 [pii]
AID - M116.734186 [pii]
AID - 10.1074/jbc.M116.734186 [doi]
PST - ppublish
SO  - J Biol Chem. 2016 Aug 26;291(35):18058-18071. doi: 10.1074/jbc.M116.734186. Epub 
      2016 Jul 5.