PMID- 27382971
OWN - NLM
STAT- MEDLINE
DCOM- 20180116
LR  - 20180116
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 22
DP  - 2016 Sep
TI  - Black phosphorus-assisted laser desorption ionization mass spectrometry for the 
      determination of low-molecular-weight compounds in biofluids.
PG  - 6223-33
LID - 10.1007/s00216-016-9737-z [doi]
AB  - Quantitative analysis of small molecules by matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been a 
      challenging task due to matrix-derived interferences in low m/z region and poor 
      reproducibility of MS signal response. In this study, we developed an approach by 
      applying black phosphorus (BP) as a matrix-assisted laser desorption ionization 
      (MALDI) matrix for the quantitative analysis of small molecules for the first 
      time. Black phosphorus-assisted laser desorption/ionization mass spectrometry 
      (BP/ALDI-MS) showed clear background and exhibited superior detection sensitivity 
      toward quaternary ammonium compounds compared to carbon-based materials. By 
      combining stable isotope labeling (SIL) strategy with BP/ALDI-MS 
      (SIL-BP/ALDI-MS), a variety of analytes labeled with quaternary ammonium group 
      were sensitively detected. Moreover, the isotope-labeled forms of analytes also 
      served as internal standards, which broadened the analyte coverage of BP/ALDI-MS 
      and improved the reproducibility of MS signals. Based on these advantages, a 
      reliable method for quantitative analysis of aldehydes from complex biological 
      samples (saliva, urine, and serum) was successfully established. Good linearities 
      were obtained for five aldehydes in the range of 0.1-20.0 muM with correlation 
      coefficients (R (2)) larger than 0.9928. The LODs were found to be 20 to 100 nM. 
      Reproducibility of the method was obtained with intra-day and inter-day relative 
      standard deviations (RSDs) less than 10.4 %, and the recoveries in saliva samples 
      ranged from 91.4 to 117.1 %. Taken together, the proposed SIL-BP/ALDI-MS strategy 
      has proved to be a reliable tool for quantitative analysis of aldehydes from 
      complex samples. Graphical Abstract An approach for the determination of small 
      molecules was developed by using black phosphorus (BP) as a matrix-assisted laser 
      desorption ionization (MALDI) matrix.
FAU - He, Xiao-Mei
AU  - He XM
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, 
      China.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, 
      China. jding@whu.edu.cn.
FAU - Yu, Lei
AU  - Yu L
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, 
      China.
FAU - Hussain, Dilshad
AU  - Hussain D
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, 
      China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, 
      China. yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160706
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Aldehydes)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Aldehydes/analysis/*blood/*urine
MH  - Humans
MH  - Isotope Labeling/methods
MH  - Limit of Detection
MH  - Phosphorus/*chemistry
MH  - Saliva/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
OTO - NOTNLM
OT  - Black phosphorus
OT  - MALDI matrix
OT  - Quantitative analysis
OT  - Quaternary ammonium compounds
OT  - Stable isotope labeling
EDAT- 2016/07/08 06:00
MHDA- 2018/01/18 06:00
CRDT- 2016/07/08 06:00
PHST- 2016/05/26 00:00 [received]
PHST- 2016/06/21 00:00 [accepted]
PHST- 2016/06/15 00:00 [revised]
PHST- 2016/07/08 06:00 [entrez]
PHST- 2016/07/08 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
AID - 10.1007/s00216-016-9737-z [pii]
AID - 10.1007/s00216-016-9737-z [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Sep;408(22):6223-33. doi: 10.1007/s00216-016-9737-z. Epub 
      2016 Jul 6.