PMID- 27383296
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 20
IP  - 12
DP  - 2016 Jun
TI  - Epiregulin-loaded PLGA nanoparticles increase human keratinocytes proliferation: 
      preliminary data.
PG  - 2484-90
LID - 11001 [pii]
AB  - OBJECTIVE: Epiregulin is a member of the epidermal growth factor (EGF) family 
      produced by keratinocytes: the aim of this study was to investigate the ability 
      of biocompatible nanoparticles loaded with such growth factor to increase human 
      keratinocytes proliferation. MATERIALS AND METHODS: Different PLGA 
      (Poly-d,l-lactide-co-glycolide)-nanoparticles (NPs) formulations have been 
      characterized in size and zeta potential by dynamic light scattering (DLS) 
      analysis. The ability of the different PLGA-NPs formulations to adhere onto 
      dental surfaces has been tested, and epiregulin-enriched PLGA-NPs has been 
      produced. Epiregulin release from NPs has been tested by enzyme-linked 
      immunosorbent (ELISA) assay and the proliferative effects of epiregulin-NPs on 
      human keratinocytes have been evaluated. RESULTS: DLS analysis revealed a 
      different size distribution depending on the PLA/PGA (poly lactic acid/poly 
      glycolic acid) ratio used. 50:50 PLGA-NPs exhibited the smaller size and the best 
      dental adhesive ability. Moreover, such epiregulin-loaded NPs was able to 
      increase cell proliferation. CONCLUSIONS: Direct dental pocket drug delivery 
      implies the NPs solution loading onto the dental surface at the cement-enamel 
      junction level: 50:50 PLGA-NPs, with their small size and excellent adhesive 
      ability, represent an interesting tool to deliver epiregulin directly where there 
      is the need for epithelial proliferation. These results describe a possible 
      strategy for periodontal pocket delivery of Epiregulin-loaded PLGA-NPs and might 
      provide a new approach for the treatment of gingival recession, where gingival 
      epithelium proliferation is needed.
FAU - Rizzi, M
AU  - Rizzi M
AD  - Innovative Research Laboratory for Wound Healing, Health Sciences Department, 
      University of Eastern Piedmont, Novara, Italy. filippo.reno@med.uniupo.it.
FAU - Migliario, M
AU  - Migliario M
FAU - Rocchetti, V
AU  - Rocchetti V
FAU - Tonello, S
AU  - Tonello S
FAU - Reno, F
AU  - Reno F
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Epiregulin)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Cell Proliferation/*drug effects
MH  - *Drug Delivery Systems
MH  - *Epiregulin/pharmacology
MH  - Humans
MH  - *Keratinocytes/cytology/drug effects
MH  - *Lactic Acid/pharmacology
MH  - Nanoparticles
MH  - *Polyglycolic Acid/pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
EDAT- 2016/07/08 06:00
MHDA- 2017/08/22 06:00
CRDT- 2016/07/08 06:00
PHST- 2016/07/08 06:00 [entrez]
PHST- 2016/07/08 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
AID - 11001 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2016 Jun;20(12):2484-90.