PMID- 27384607
OWN - NLM
STAT- MEDLINE
DCOM- 20170427
LR  - 20181113
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 65
IP  - 10
DP  - 2016 Oct
TI  - The modulation of Th2 immune pathway in the immunosuppressive effect of human 
      umbilical cord mesenchymal stem cells in a murine asthmatic model.
PG  - 795-801
LID - 10.1007/s00011-016-0961-y [doi]
AB  - BACKGROUND: Asthma is a chronic airway inflammatory disease that has a high 
      prevalence nowadays, and seeking the means of relieving asthmatic symptoms is now 
      an issue with increased importance. While mesenchymal stem cells have been 
      demonstrated to display immunomodulatory effects, the effect of fetus-type 
      mesenchymal stem cells (MSCs) on asthmatic symptoms in vivo have not been 
      reported to date. METHODS: Female BALB/c mice at 8 weeks of age were sensitized 
      by ovalbumin, and MSCs derived from Wharton's jelly of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) were injected into the asthmatic mice. Airway 
      hyper-responsiveness, lung eosinophil infiltration, cytokine level in splenocyte 
      cultures and serum immunoglobulin level were measured. Enzyme-linked 
      immunosorbent assay was used to determine cytokine and immunoglobulin levels. 
      RESULTS: This current study demonstrated that hUCMSCs attenuated both lung 
      lymphocyte and eosinophil infiltration, and significantly decreased the 
      concentration of Th2 cytokines interleukin-5 in splenocyte cultures. CONCLUSIONS: 
      Human umbilical cord mesenchymal stem cells have the advantage of being easily 
      harvested non-invasively and are capable of rapid proliferation, therefore an 
      ideal material for stem cell-based immune therapies. The current study showed 
      that fetal-type MSCs were able to suppress asthmatic symptoms efficiently, and 
      its immunomodulatory effect resulted primarily from suppressing the Th2 pathway 
      in the animal model. This study suggested that hUCMSCs could be an ideal 
      candidate for cell-based therapies of asthma.
FAU - Chan, Chin-Kan
AU  - Chan CK
AD  - Department of Pediatrics, Taoyuan General Hospital, Taoyuan, Taiwan.
AD  - Hsin Sheng Junior College of Medical Care and Management, Taoyuan, Taiwan.
FAU - Lin, Ting-Chun
AU  - Lin TC
AD  - Division of Allergy, Asthma and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital and College of Medicine, Chang Gung Memorial Hospital at 
      Linkou, No. 5, Fu-Hsin Street, Kweishan, Taoyuan, Taiwan.
AD  - School of Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Huang, Yung-An
AU  - Huang YA
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Chen, Ya-Shan
AU  - Chen YS
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Wu, Chia-Ling
AU  - Wu CL
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Lo, Huei-Yu
AU  - Lo HY
AD  - Department of Gynaecology, Taoyuan General Hospital, Taoyuan, Taiwan.
FAU - Kuo, Ming-Ling
AU  - Kuo ML
AD  - Division of Allergy, Asthma and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital and College of Medicine, Chang Gung Memorial Hospital at 
      Linkou, No. 5, Fu-Hsin Street, Kweishan, Taoyuan, Taiwan.
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
AD  - Department of Microbiology and Immunology, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Wu, Kang-Hsi
AU  - Wu KH
AD  - Department of Pediatrics, Children's Hospital and School of Chinese Medicine, 
      China Medical University and Hospitals, Taichung, Taiwan. d5284@mail.cmuh.org.tw.
FAU - Huang, Jing-Long
AU  - Huang JL
AD  - Division of Allergy, Asthma and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital and College of Medicine, Chang Gung Memorial Hospital at 
      Linkou, No. 5, Fu-Hsin Street, Kweishan, Taoyuan, Taiwan. long@adm.cgmh.org.tw.
LA  - eng
PT  - Journal Article
DEP - 20160706
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens
MH  - Animals
MH  - Asthma/immunology/*therapy
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cytokines/immunology
MH  - Disease Models, Animal
MH  - Eosinophils/immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Leukocyte Count
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice, Inbred BALB C
MH  - Ovalbumin
MH  - Spleen/cytology
MH  - Th2 Cells/immunology
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - Allergic symptoms
OT  - Asthma
OT  - MSC
OT  - Mesenchymal stem cell
OT  - Mice
OT  - Murine
OT  - Umbilical cord
OT  - Wharton's jelly
EDAT- 2016/07/08 06:00
MHDA- 2017/04/28 06:00
CRDT- 2016/07/08 06:00
PHST- 2015/06/23 00:00 [received]
PHST- 2016/06/11 00:00 [accepted]
PHST- 2016/06/03 00:00 [revised]
PHST- 2016/07/08 06:00 [entrez]
PHST- 2016/07/08 06:00 [pubmed]
PHST- 2017/04/28 06:00 [medline]
AID - 10.1007/s00011-016-0961-y [pii]
AID - 10.1007/s00011-016-0961-y [doi]
PST - ppublish
SO  - Inflamm Res. 2016 Oct;65(10):795-801. doi: 10.1007/s00011-016-0961-y. Epub 2016 
      Jul 6.