PMID- 27385599
OWN - NLM
STAT- MEDLINE
DCOM- 20170619
LR  - 20180301
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 56
IP  - 9
DP  - 2016 Sep
TI  - Leukoreduced red blood cell transfusions do not induce platelet glycoprotein 
      antibodies in patients with sickle cell disease.
PG  - 2267-73
LID - 10.1111/trf.13693 [doi]
AB  - BACKGROUND: Alloimmunization to red blood cell (RBC) antigens after transfusion 
      is well described in patients with sickle cell disease (SCD). We recently 
      demonstrated that leukocyte-reduced RBC transfusions appeared to induce human 
      leukocyte antigen (HLA) antibodies in some children with SCD; now, we hypothesize 
      that residual platelets contained in transfused RBC products may lead to platelet 
      glycoprotein antibody formation. STUDY DESIGN AND METHODS: A cross-sectional 
      study was conducted among never pregnant pediatric patients with SCD who either 
      had received many RBC transfusions or had never received any transfusions. Serum 
      was tested for antibodies to platelet-specific glycoproteins using a commercial 
      enzyme immunoassay. RESULTS: Platelet-specific glycoprotein antibodies were found 
      in 12 of 90 patients (13%) in the transfused group versus 5 of 24 patients (21%) 
      in the never transfused group (p = 0.35). The prevalence of antibodies as well as 
      the median standardized optical density for these two groups was not 
      significantly different for any of the studied platelet glycoprotein antigens. 
      There was no association with the presence of platelet-specific glycoprotein 
      antibodies with either RBC or HLA antibodies. CONCLUSIONS: Leukocyte-reduced RBC 
      transfusions do not appear to induce platelet-specific glycoprotein antibodies. 
      The positive platelet-specific glycoprotein antibody results from this study may 
      represent platelet autoantibodies, platelet alloantibodies, or false-positive 
      reactions. A better understanding of the immunobiology of patients with SCD at 
      baseline and after blood product exposure may help improve future transfusion and 
      transplantation.
CI  - (c) 2016 AABB.
FAU - Nickel, Robert Sheppard
AU  - Nickel RS
AD  - Division of Hematology, Children's National Health System, Washington, DC. 
      rnickel@childrensnational.org.
FAU - Winkler, Anne M
AU  - Winkler AM
AD  - Department of Pathology and Laboratory Medicine, Center for Transfusion and 
      Cellular Therapy, Children's Healthcare of Atlanta, Emory University, Atlanta, 
      Georgia.
FAU - Horan, John T
AU  - Horan JT
AD  - Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, Emory 
      University, Atlanta, Georgia.
FAU - Hendrickson, Jeanne E
AU  - Hendrickson JE
AD  - Department of Pediatrics, Yale University, New Haven, Connecticut.
AD  - Department of Laboratory Medicine, Yale University, New Haven, Connecticut.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160706
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antibodies)
RN  - 0 (Autoantibodies)
RN  - 0 (Isoantibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Anemia, Sickle Cell/*blood
MH  - Antibodies/*blood/*immunology
MH  - Autoantibodies/immunology
MH  - Blood Platelets/immunology
MH  - Cross-Sectional Studies
MH  - Erythrocyte Transfusion/*methods
MH  - Humans
MH  - Isoantibodies/immunology
MH  - Platelet Membrane Glycoproteins/*immunology
EDAT- 2016/07/08 06:00
MHDA- 2017/06/20 06:00
CRDT- 2016/07/08 06:00
PHST- 2016/03/01 00:00 [received]
PHST- 2016/05/10 00:00 [revised]
PHST- 2016/05/10 00:00 [accepted]
PHST- 2016/07/08 06:00 [entrez]
PHST- 2016/07/08 06:00 [pubmed]
PHST- 2017/06/20 06:00 [medline]
AID - 10.1111/trf.13693 [doi]
PST - ppublish
SO  - Transfusion. 2016 Sep;56(9):2267-73. doi: 10.1111/trf.13693. Epub 2016 Jul 6.