PMID- 27387861
OWN - NLM
STAT- MEDLINE
DCOM- 20170519
LR  - 20181113
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 186
IP  - 2
DP  - 2016 Aug
TI  - A Molecular Profile of the Endothelial Cell Response to Ionizing Radiation.
PG  - 141-52
LID - 10.1667/RR14444.1 [doi]
AB  - Ionizing radiation exposure can cause acute radiation sickness (ARS) by damaging 
      the hematopoietic compartment. Radiation damages quiescent hematopoietic stem 
      cells (HSCs) and proliferating hematopoietic cells, resulting in neutropenia, 
      thrombocytopenia and increased risk for long-term hematopoietic dysfunction and 
      myelodysplasia. While some aspects of the hematopoietic response to radiation 
      injury are intrinsic to hematopoietic cells, the recovery of the HSC pool and 
      overall hematopoiesis is also dependent on signals from bone marrow endothelial 
      cells (BM ECs) within the HSC vascular niche. The precise mechanisms through 
      which BM ECs regulate HSC regeneration remain unclear. Characterization of the 
      altered EC gene expression that occurs in response to radiation could provide a 
      roadmap to the discovery of EC-derived mechanisms that regulate hematopoietic 
      regeneration. Here, we show that 5 Gy total-body irradiation substantially alters 
      the expression of numerous genes in BM ECs within 24 h and this molecular 
      response largely resolves by day 14 postirradiation. Several unique and 
      nonannotated genes, which encode secreted proteins were upregulated and 
      downregulated in ECs in response to radiation. These results highlight the 
      complexity of the molecular response of BM ECs to ionizing radiation and identify 
      several candidate mechanisms that should be prioritized for functional analysis 
      in models of hematopoietic injury and regeneration.
FAU - Himburg, Heather A
AU  - Himburg HA
AD  - a Division of Hematology/Oncology, Department of Medicine.
FAU - Sasine, Joshua
AU  - Sasine J
AD  - a Division of Hematology/Oncology, Department of Medicine.
FAU - Yan, Xiao
AU  - Yan X
AD  - a Division of Hematology/Oncology, Department of Medicine.
FAU - Kan, Jenny
AU  - Kan J
AD  - a Division of Hematology/Oncology, Department of Medicine.
FAU - Dressman, Holly
AU  - Dressman H
AD  - d Center for Bioinformatics and Genetics, Duke University, Durham, North 
      Carolina.
FAU - Chute, John P
AU  - Chute JP
AD  - a Division of Hematology/Oncology, Department of Medicine.
AD  - b Jonsson Comprehensive Cancer Center and.
AD  - c Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, 
      University of California, Los Angeles, Los Angeles, California; and.
LA  - eng
GR  - U01 AI107333/AI/NIAID NIH HHS/United States
GR  - U19 AI067769/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160707
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Death/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Endothelial Cells/*cytology/metabolism/*radiation effects
MH  - Female
MH  - Gene Expression Regulation/radiation effects
MH  - Mice
MH  - Radiation, Ionizing
MH  - Time Factors
MH  - Whole-Body Irradiation/adverse effects
PMC - PMC6016841
MID - NIHMS810981
EDAT- 2016/07/09 06:00
MHDA- 2017/05/20 06:00
PMCR- 2018/06/25
CRDT- 2016/07/09 06:00
PHST- 2016/07/09 06:00 [entrez]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2017/05/20 06:00 [medline]
PHST- 2018/06/25 00:00 [pmc-release]
AID - 10.1667/RR14444.1 [doi]
PST - ppublish
SO  - Radiat Res. 2016 Aug;186(2):141-52. doi: 10.1667/RR14444.1. Epub 2016 Jul 7.