PMID- 27388771
OWN - NLM
STAT- MEDLINE
DCOM- 20170210
LR  - 20231111
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 11
IP  - 1
DP  - 2016 Jul 7
TI  - Frequent detection of human polyomavirus 6 in keratoacanthomas.
PG  - 58
LID - 10.1186/s13000-016-0509-z [doi]
LID - 58
AB  - BACKGROUND: The recent discovery of the Merkel cell polyomavirus and its 
      consistent association with Merkel cell carcinoma has drawn attention to the 
      numerous recently discovered polyomaviruses and their possible involvement in the 
      etiopathogenesis of non-melanoma skin cancer (NMSC). Data on the recently 
      discovered human polyomavirus 6 (HPyV6) and its role in NMSC are sparse and in 
      part controversial. METHODS: In the present study we tested a large number 
      (n = 299) of NMSC specimens for the presence of human polyomavirus 6 (HPyV6) by 
      DNA PCR and HPyV6 fluorescence in situ hybridization (FISH). In detail, 59 
      keratoacanthomas (KA), 109 basal cell carcinomas (BCC), 86 squamous cell 
      carcinomas (SCC) and 45 trichoblastomas (TB) were tested for the presence of 
      HPyV6. RESULTS: HPyV6 DNA PCR and subsequent sequence analysis revealed that 25 
      KAs (42.3 %), 23 BCCs (21.1 %), 8 SCCs (9.3 %) and 10 TBs (22.2 %) were HPyV6 
      positive. The presence of HPyV6 DNA was visualized and validated on the single 
      cell level within the histomorphological context by HPyV6 fluorescence in situ 
      hybridization. CONCLUSIONS: The high frequency of HPyV6 DNA in 42.3 % of KA 
      possibly points to a role for HPyV6 in the etiopathogenesis of KAs. Although the 
      detection rate of HPyV6 DNA in BCCs and TBs is within the previously reported 
      detection range in normal skin, it does not exclude a possible role for HPyV6 in 
      the carcinogenesis in a significant subset of these skin tumors.
FAU - Beckervordersandforth, Jan
AU  - Beckervordersandforth J
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Pujari, Sreedhar
AU  - Pujari S
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Rennspiess, Dorit
AU  - Rennspiess D
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Speel, Ernst Jan M
AU  - Speel EJ
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Winnepenninckx, Veronique
AU  - Winnepenninckx V
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Diaz, Carlos
AU  - Diaz C
AD  - Center for Dermatopathology, Freiburg, Germany.
FAU - Weyers, Wolfgang
AU  - Weyers W
AD  - Center for Dermatopathology, Freiburg, Germany.
FAU - Haugg, Anke Maria
AU  - Haugg AM
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands.
FAU - Kurz, Anna Kordelia
AU  - Kurz AK
AD  - Medizinische Klinik IV, Universitatsklinikum Aachen, Aachen, Germany.
FAU - Zur Hausen, Axel
AU  - Zur Hausen A
AD  - Department of Pathology, GROW-School for Oncology & Developmental Biology, 
      Maastricht University Medical Center, P. Debyelaan 25, P.O. Box 5800, 6202 AZ, 
      Maastricht, The Netherlands. axel.zurhausen@mumc.nl.
LA  - eng
PT  - Journal Article
DEP - 20160707
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Carcinoma, Merkel Cell/*virology
MH  - Carcinoma, Squamous Cell/*virology
MH  - Cohort Studies
MH  - DNA, Viral/genetics
MH  - Germany
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratoacanthoma/*virology
MH  - Merkel cell polyomavirus/genetics/isolation & purification
MH  - Polymerase Chain Reaction
MH  - Polyomavirus/genetics/*isolation & purification
MH  - Sequence Analysis, DNA
MH  - Skin/pathology
MH  - Skin Neoplasms/*virology
PMC - PMC4936261
OTO - NOTNLM
OT  - FISH
OT  - Fluorescence in situ hybridization
OT  - HPyV6
OT  - Human Polyomavirus 6
OT  - Keratoacanthoma
OT  - Non melanoma skin cancer
EDAT- 2016/07/09 06:00
MHDA- 2017/02/12 06:00
PMCR- 2016/07/07
CRDT- 2016/07/09 06:00
PHST- 2015/11/16 00:00 [received]
PHST- 2016/06/29 00:00 [accepted]
PHST- 2016/07/09 06:00 [entrez]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2017/02/12 06:00 [medline]
PHST- 2016/07/07 00:00 [pmc-release]
AID - 10.1186/s13000-016-0509-z [pii]
AID - 509 [pii]
AID - 10.1186/s13000-016-0509-z [doi]
PST - epublish
SO  - Diagn Pathol. 2016 Jul 7;11(1):58. doi: 10.1186/s13000-016-0509-z.