PMID- 27390099
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230120
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 54
IP  - 9
DP  - 2016 Oct 17
TI  - Simultaneous Determination of beta-Cypermethrin and Its Metabolite 3-Phenoxybenzoic 
      Acid in Microbial Degradation Systems by HPLC-UV.
PG  - 1584-1592
LID - 10.1093/chromsci/bmw108 [doi]
AB  - The wide use of pesticides in agriculture is necessary to guarantee adequate food 
      production worldwide. However, pesticide residues have caused global concern 
      because of their potential health risk to consumers. In this study, we could 
      identify beta-cypermethrin (beta-CY) and its degradation product 3-phenoxybenzoic acid 
      (3-PBA) by liquid chromatograph-mass spectrometry. Few studies on the 
      simultaneous determination of beta-CY and its metabolites have been carried out so 
      far; hence, we established a high-performance liquid chromatography method to 
      determine the concentrations of both beta-CY and 3-PBA simultaneously in microbial 
      degradation systems. In this study, a novel beta-CY degrading strain, Bacillus 
      licheniformis B-1, was isolated from a tea garden soil, utilizing beta-CY as a 
      growth substrate. Good linear relationships between beta-CY and 3-PBA were observed 
      and the concentrations of reference solutions were between 0.50 and 60.00 microg/mL. 
      Satisfactory stability and intra- and interday precision were obtained. The 
      limits of detection were 0.06 and 0.13 microg/mL for beta-CY and 3-PBA, respectively, 
      and the corresponding limits of quantification were 0.21 and 0.34 microg/mL, 
      respectively. Spiking recoveries for beta-CY varied from 98.38 to 105.80%, with 
      relative standard deviations (RSDs) varying from 1.49 to 3.93%. Spiking 
      recoveries for 3-PBA varied from 99.59 to 101.20%, with RSDs varying from 0.58 to 
      3.64%. The proposed method has advantages of simplicity, rapidity, high accuracy, 
      good separation and reproducibility; thus, it is ideally suitable for 
      simultaneous determination of beta-CY and 3-PBA in microbial degradation systems.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Li, Jianlong
AU  - Li J
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
AD  - Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China.
FAU - Lin, Derong
AU  - Lin D
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Ji, Ran
AU  - Ji R
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Yao, Kai
AU  - Yao K
AD  - College of Light Industry and Food, Sichuan University, Chengdu, Sichuan 610065, 
      P.R. China.
FAU - Deng, Wei-Qin
AU  - Deng WQ
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Yuan, Huaiyu
AU  - Yuan H
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Wu, Qi
AU  - Wu Q
AD  - College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P. R. China.
FAU - Jia, Qiusi
AU  - Jia Q
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Luo, Peiwen
AU  - Luo P
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Zhou, Kang
AU  - Zhou K
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - He, Li
AU  - He L
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
FAU - Zou, Likou
AU  - Zou L
AD  - The Laboratory of Microbiology, Dujiangyan Campus, Sichuan Agricultural 
      University, Dujiangyan, Sichuan 611830, P.R. China.
FAU - Liu, Shuliang
AU  - Liu S
AD  - College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, 
      P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
SB  - IM
EDAT- 2016/07/09 06:00
MHDA- 2016/07/09 06:01
CRDT- 2016/07/09 06:00
PHST- 2014/12/11 00:00 [received]
PHST- 2016/03/30 00:00 [revised]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2016/07/09 06:01 [medline]
PHST- 2016/07/09 06:00 [entrez]
AID - bmw108 [pii]
AID - 10.1093/chromsci/bmw108 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2016 Oct 17;54(9):1584-1592. doi: 10.1093/chromsci/bmw108.