PMID- 27390910
OWN - NLM
STAT- MEDLINE
DCOM- 20180727
LR  - 20181202
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 10
IP  - 8
DP  - 2016 Aug 23
TI  - Effective Delivery of Antigen-Encapsulin Nanoparticle Fusions to Dendritic Cells 
      Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection.
PG  - 7339-50
LID - 10.1021/acsnano.5b08084 [doi]
AB  - In cancer immunotherapy, robust and efficient activation of cytotoxic CD8(+) T 
      cell immune responses is a promising, but challenging task. Dendritic cells (DCs) 
      are well-known professional antigen presenting cells that initiate and regulate 
      antigen-specific cytotoxic CD8(+) T cells that kill their target cells directly 
      as well as secrete IFN-gamma, a cytokine critical in tumor rejection. Here, we 
      employed recently established protein cage nanoparticles, encapsulin (Encap), as 
      antigenic peptide nanocarriers by genetically incorporating the OT-1 peptide of 
      ovalbumin (OVA) protein to the three different positions of the Encap subunit. 
      With them, we evaluated their efficacy in activating DC-mediated antigen-specific 
      T cell cytotoxicity and consequent melanoma tumor rejection in vivo. DCs 
      efficiently engulfed Encap and its variants (OT-1-Encaps), which carry antigenic 
      peptides at different positions, and properly processed them within phagosomes. 
      Delivered OT-1 peptides were effectively presented by DCs to naive CD8(+) T cells 
      successfully, resulting in the proliferation of antigen-specific cytotoxic CD8(+) 
      T cells. OT-1-Encap vaccinations in B16-OVA melanoma tumor bearing mice 
      effectively activated OT-1 peptide specific cytotoxic CD8(+) T cells before or 
      even after tumor generation, resulting in significant suppression of tumor growth 
      in prophylactic as well as therapeutic treatments. A large number of cytotoxic 
      CD8(+) T cells that actively produce both intracellular and secretory IFN-gamma were 
      observed in tumor-infiltrating lymphocytes collected from B16-OVA tumor masses 
      originally vaccinated with OT-1-Encap-C upon tumor challenges. The approaches we 
      describe herein may provide opportunities to develop epitope-dependent 
      vaccination systems that stimulate and/or modulate efficient and epitope-specific 
      cytotoxic T cell immune responses in nonpathogenic diseases.
FAU - Choi, Bongseo
AU  - Choi B
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
FAU - Moon, Hyojin
AU  - Moon H
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
FAU - Hong, Sung Joon
AU  - Hong SJ
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
FAU - Shin, Changsik
AU  - Shin C
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
FAU - Do, Yoonkyung
AU  - Do Y
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
FAU - Ryu, Seongho
AU  - Ryu S
AD  - Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University , 
      Cheonan, 336-745, Korea.
FAU - Kang, Sebyung
AU  - Kang S
AD  - Department of Biological Sciences, School of Life Sciences, Ulsan National 
      Institute of Science and Technology (UNIST) , Ulsan, 689-798, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160713
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Drug Carriers)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes
MH  - *Dendritic Cells
MH  - *Drug Carriers
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Nanoparticles
MH  - Ovalbumin
MH  - *T-Lymphocytes, Cytotoxic
OTO - NOTNLM
OT  - antigen delivery
OT  - cytotoxic T cells
OT  - dendritic cells
OT  - encapsulin
OT  - vaccination
EDAT- 2016/07/09 06:00
MHDA- 2018/07/28 06:00
CRDT- 2016/07/09 06:00
PHST- 2016/07/09 06:00 [entrez]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2018/07/28 06:00 [medline]
AID - 10.1021/acsnano.5b08084 [doi]
PST - ppublish
SO  - ACS Nano. 2016 Aug 23;10(8):7339-50. doi: 10.1021/acsnano.5b08084. Epub 2016 Jul 
      13.