PMID- 27391069
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20201218
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 31
DP  - 2016 Aug 2
TI  - P2X7 targeting inhibits growth of human mesothelioma.
PG  - 49664-49676
LID - 10.18632/oncotarget.10430 [doi]
AB  - Malignant pleural mesothelioma (MPM) is an aggressive tumor refractory to 
      anti-blastic therapy. MPM cells show several genetic and biochemical defects, 
      e.g. overexpression of oncogenes, downregulation of onco-suppressor genes, 
      dysregulation of microRNA, or alteration of intracellular Ca2+ homeostasis and of 
      apoptosis. No information is as yet available on purinergic signalling in this 
      tumor. Signalling via the P2X7 (P2RX7 or P2X7R) purinergic receptor is attracting 
      increasing attention as a pathway involved in cancer cell death or proliferation. 
      In this report we show that the P2X7R is expressed by three MPM cell lines 
      established from MPM patients but not by mesothelial cells from healthy subjects 
      (healthy mesothelial cells, HMCs). MPM cell proliferation was inhibited by in 
      vitro incubation in the presence of selective P2X7R antagonists, as well as by 
      stimulation with the P2X7R agonist BzATP. Systemic administration of the 
      selective P2X7R blocker AZ10606120 inhibited in vivo growth of MPM tumors whether 
      implanted subcutaneously (s.c.) or intraperitoneally (i.p.). Our findings suggest 
      that the P2X7R might be a novel target for the therapy of mesothelioma.
FAU - Amoroso, Francesca
AU  - Amoroso F
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Salaro, Erica
AU  - Salaro E
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Falzoni, Simonetta
AU  - Falzoni S
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Chiozzi, Paola
AU  - Chiozzi P
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Giuliani, Anna Lisa
AU  - Giuliani AL
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Cavallesco, Giorgio
AU  - Cavallesco G
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Maniscalco, Pio
AU  - Maniscalco P
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Puozzo, Andrea
AU  - Puozzo A
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Bononi, Ilaria
AU  - Bononi I
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Martini, Fernanda
AU  - Martini F
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Tognon, Mauro
AU  - Tognon M
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
FAU - Di Virgilio, Francesco
AU  - Di Virgilio F
AD  - Department of Morphology, Surgery and Experimental Medicine, University of 
      Ferrara, Ferrara, Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (MicroRNAs)
RN  - 0 (Purinergic P2X Receptor Antagonists)
RN  - 0 (Receptors, Purinergic P2X7)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Apoptosis
MH  - Biopsy
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cytoplasm/metabolism
MH  - Humans
MH  - Lung Neoplasms/*metabolism
MH  - Male
MH  - Mesothelioma/*metabolism
MH  - Mesothelioma, Malignant
MH  - Mice
MH  - Mice, Nude
MH  - MicroRNAs/metabolism
MH  - Pleural Neoplasms/*metabolism
MH  - Purinergic P2X Receptor Antagonists/*pharmacology
MH  - Receptors, Purinergic P2X7/*metabolism
MH  - Signal Transduction
PMC - PMC5226537
OTO - NOTNLM
OT  - P2X7
OT  - cancer
OT  - extracellular ATP
OT  - mesothelioma
OT  - purinergic signalling
COIS- The Authors declare no conflicts of interest.
EDAT- 2016/07/09 06:00
MHDA- 2018/02/24 06:00
PMCR- 2016/08/02
CRDT- 2016/07/09 06:00
PHST- 2015/12/16 00:00 [received]
PHST- 2016/06/26 00:00 [accepted]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2016/07/09 06:00 [entrez]
PHST- 2016/08/02 00:00 [pmc-release]
AID - 10430 [pii]
AID - 10.18632/oncotarget.10430 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Aug 2;7(31):49664-49676. doi: 10.18632/oncotarget.10430.