PMID- 27391207
OWN - NLM
STAT- MEDLINE
DCOM- 20170607
LR  - 20191210
IS  - 1520-6777 (Electronic)
IS  - 0733-2467 (Linking)
VI  - 36
IP  - 4
DP  - 2017 Apr
TI  - Effect of urethral wall injection of replication-defective herpes simplex 
      virus-mediated gene transfer of kynurenine aminotransferase on urethral pressure 
      in spinal cord-injured rats.
PG  - 1046-1051
LID - 10.1002/nau.23067 [doi]
AB  - AIMS: We determined whether or not replication-defective herpes simplex virus 
      vector-mediated kynurenine aminotransferase II (HSVrd-KAT II) suppressed the 
      tonic activity of the urethral sphincter in spinal cord-injured (SCI) rats. 
      METHODS: Thirty-six adult female Sprague-Dawley rats were used to produce a 
      spinal cord injury model. One week after spinalization, HSVrd-KAT II was injected 
      into the urethral wall of rats and another two groups of SCI rats were treated 
      with saline and HSVrd as controls. Three weeks after viral injection, the 
      urethral pressure profile (UPP), continuous cystometry, and gene expression in 
      the L6-S1 spinal cords were evaluated in all three groups. RESULTS: In the 
      HSVrd-KAT II group, the maximum urethral closure pressure (Pclo.max) and maximum 
      voiding pressure were significantly decreased (23.6-24.9% and 31.6-30.9%, 
      respectively), in addition to an increase in voiding efficiency(48.8-76%), 
      compared with the sham and HSVrd groups. The KAT II protein and mRNA levels were 
      significantly increased in HSV-KAT II group compared with the HSVrd group. 
      CONCLUSION: KAT II gene therapy effectively reduced the urethral pressure, 
      improving detrusor-sphincter dyssynergia (DSD), and detrusor overactivity (DO), 
      probably by blocking the N-methyl-D-aspartate receptor (NMDAr) in the L6-S1 
      spinal cord. Neurourol. Urodynam. 36:1046-1051, 2017. (c) 2016 Wiley Periodicals, 
      Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Wang, Zhaoxia
AU  - Wang Z
AD  - Department of Urology, China Rehabilitation Research Centre, Rehabilitation 
      School of Capital Medical University (CMU), Beijing, China.
AD  - Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, 
      Beijing, China.
AD  - Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China.
FAU - Liao, Limin
AU  - Liao L
AD  - Department of Urology, China Rehabilitation Research Centre, Rehabilitation 
      School of Capital Medical University (CMU), Beijing, China.
AD  - Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, 
      Beijing, China.
AD  - Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160708
PL  - United States
TA  - Neurourol Urodyn
JT  - Neurourology and urodynamics
JID - 8303326
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.7 (kynurenine-oxoglutarate transaminase)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - *Gene Transfer Techniques
MH  - *Genetic Vectors
MH  - Injections
MH  - Pressure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Simplexvirus
MH  - Transaminases/*administration & dosage
MH  - *Urethra/physiopathology
MH  - Urinary Bladder
MH  - Urinary Bladder, Neurogenic/etiology/therapy
MH  - Urination Disorders/etiology/*therapy
MH  - Urodynamics
OTO - NOTNLM
OT  - KAT II
OT  - NMDAr
OT  - detrusor overactivity
OT  - detrusor-sphincter dyssynergia
OT  - gene therapy
OT  - herpes simple virus
OT  - spinal cord injury
EDAT- 2016/07/09 06:00
MHDA- 2017/06/08 06:00
CRDT- 2016/07/09 06:00
PHST- 2016/04/18 00:00 [received]
PHST- 2016/06/22 00:00 [accepted]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2017/06/08 06:00 [medline]
PHST- 2016/07/09 06:00 [entrez]
AID - 10.1002/nau.23067 [doi]
PST - ppublish
SO  - Neurourol Urodyn. 2017 Apr;36(4):1046-1051. doi: 10.1002/nau.23067. Epub 2016 Jul 
      8.