PMID- 27391447
OWN - NLM
STAT- MEDLINE
DCOM- 20170720
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 7
DP  - 2016
TI  - Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of 
      Triticum polonicum L.
PG  - e0158883
LID - 10.1371/journal.pone.0158883 [doi]
LID - e0158883
AB  - Triticum genus encloses several tetraploid species that are used as genetic 
      stocks for expanding the genetic variability of wheat (Triticum aestivum L.). 
      Although the T. aestivum (2n = 6x = 42, AABBDD) and T. durum (2n = 4x = 28, AABB) 
      karyotypes were well examined by chromosome staining, Giemsa C-banding and FISH 
      markers, other tetraploids are still poorly characterized. Here, we established 
      and compared the fluorescence in situ hybridization (FISH) patterns on 
      chromosomes of 20 accessions of T. polonicum species using different repetitive 
      sequences from BAC library of wheat 'Chinese Spring'. The chromosome patterns of 
      Polish wheat were compared to tetraploid (2n = 4x = 28, AABB) Triticum species: 
      T. durum, T. diccocon and T. turanicum, as well. A combination of pTa-86, pTa-535 
      and pTa-713 probes was the most informative among 6 DNA probes tested. Probe 
      pTa-k374, which is similar to 28S rDNA sequence enabled to distinguish signal 
      size and location differences, as well as rDNA loci elimination. Furthermore, 
      pTa-465 and pTa-k566 probes are helpful for the detection of similar organized 
      chromosomes. The polymorphisms of signals distribution were observed in 2A, 2B, 
      3B, 5B, 6A and 7B chromosomes. Telomeric region of the short arm of 6B chromosome 
      was the most polymorphic. Our work is novel and contributes to the understanding 
      of T. polonicum genome organization which is essential to develop successful 
      advanced breeding strategies for wheat. Collection and characterization of this 
      germplasm can contribute to the wheat biodiversity safeguard.
FAU - Kwiatek, Michal
AU  - Kwiatek M
AUID- ORCID: 0000-0001-9442-3124
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 
      Poznan, Poland.
FAU - Majka, Maciej
AU  - Majka M
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 
      Poznan, Poland.
FAU - Majka, Joanna
AU  - Majka J
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 
      Poznan, Poland.
FAU - Belter, Jolanta
AU  - Belter J
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 
      Poznan, Poland.
FAU - Suchowilska, Elzbieta
AU  - Suchowilska E
AD  - Department of Plant Breeding and Seed Production, University of Warmia and Mazury 
      in Olsztyn, Pl. Lodzki 3, 10-727 Olsztyn, Poland.
FAU - Wachowska, Urszula
AU  - Wachowska U
AD  - Department of Entomology, Phytopathology and Molecular Diagnostics, University of 
      Warmia and Mazury in Olsztyn, Pl. Lodzki 3, 10-727 Olsztyn, Poland.
FAU - Wiwart, Marian
AU  - Wiwart M
AD  - Department of Plant Breeding and Seed Production, University of Warmia and Mazury 
      in Olsztyn, Pl. Lodzki 3, 10-727 Olsztyn, Poland.
FAU - Wisniewska, Halina
AU  - Wisniewska H
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 
      Poznan, Poland.
LA  - eng
PT  - Journal Article
DEP - 20160708
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 28S)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - Cytogenetics
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Genetic Markers
MH  - *Polymorphism, Genetic
MH  - RNA, Plant/genetics
MH  - RNA, Ribosomal, 28S/genetics
MH  - Triticum/*genetics
PMC - PMC4938433
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/07/09 06:00
MHDA- 2017/07/21 06:00
PMCR- 2016/07/08
CRDT- 2016/07/09 06:00
PHST- 2016/04/22 00:00 [received]
PHST- 2016/06/23 00:00 [accepted]
PHST- 2016/07/09 06:00 [entrez]
PHST- 2016/07/09 06:00 [pubmed]
PHST- 2017/07/21 06:00 [medline]
PHST- 2016/07/08 00:00 [pmc-release]
AID - PONE-D-16-16286 [pii]
AID - 10.1371/journal.pone.0158883 [doi]
PST - epublish
SO  - PLoS One. 2016 Jul 8;11(7):e0158883. doi: 10.1371/journal.pone.0158883. 
      eCollection 2016.