PMID- 27393003
OWN - NLM
STAT- MEDLINE
DCOM- 20180104
LR  - 20180122
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 97
DP  - 2016 Aug
TI  - Mitochondrial Peroxiredoxin-3 protects against hyperglycemia induced myocardial 
      damage in Diabetic cardiomyopathy.
PG  - 489-500
LID - S0891-5849(16)30307-0 [pii]
LID - 10.1016/j.freeradbiomed.2016.06.019 [doi]
AB  - Mitochondrial oxidative stress has emerged as a key contributor towards the 
      development of diabetic cardiomyopathy. Peroxiredoxin-3 (Prx-3), a mitochondrial 
      antioxidant, scavenges H2O2 and offers protection against ROS related 
      pathologies. We observed a decrease in the expression of Prx-3 in the hearts of 
      streptozotocin (STZ) induced diabetic rats, and also high glucose treated H9c2 
      cardiac cells, which may augment oxidative stress mediated damage. Hence we 
      hypothesized that overexpression of Prx-3 could prevent the cardiac damage 
      associated with diabetes. In this study we used quercetin (QUE) to achieve Prx-3 
      induction in vivo, while a Prx-3 overexpressing H9c2 cell line was employed for 
      carrying out in vitro studies. Diabetes was induced in Wistar rats by a single 
      intraperitoneal injection of STZ. Quercetin (50mg/kg body weight) was delivered 
      orally to hyperglycemic and age matched control rats for 2 months. Quercetin 
      treatment induced the myocardial expression of Prx-3 but not Prx-5 both in 
      control and STZ rats. Prx-3 induction by quercetin prevented diabetes induced 
      oxidative stress as confirmed by decrease in expression of markers such as 4-HNE 
      and mitochondrial uncoupling protein, UCP-3. It was also successful in reducing 
      cardiac cell apoptosis, hypertrophy and fibrosis leading to amelioration of 
      cardiac contractility defects. Overexpression of Prx-3 in cultured H9c2 cardiac 
      cells could significantly diminish high glucose inflicted mitochondrial oxidative 
      damage and apoptosis, thus strengthening our hypothesis. These results suggest 
      that diabetes induced cardiomyopathy can be prevented by elevating Prx-3 levels 
      thereby providing extensive protection to the diabetic heart.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Arkat, Silpa
AU  - Arkat S
AD  - National Centre for Cell Science, NCCS Complex, S.P. Pune University, 
      Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address: 
      silpa.knlarkat@gmail.com.
FAU - Umbarkar, Prachi
AU  - Umbarkar P
AD  - National Centre for Cell Science, NCCS Complex, S.P. Pune University, 
      Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address: 
      prachiu37@gmail.com.
FAU - Singh, Sarojini
AU  - Singh S
AD  - National Centre for Cell Science, NCCS Complex, S.P. Pune University, 
      Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address: 
      sarojinisingh10@gmail.com.
FAU - Sitasawad, Sandhya L
AU  - Sitasawad SL
AD  - National Centre for Cell Science, NCCS Complex, S.P. Pune University, 
      Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address: 
      ssitaswad@nccs.res.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160705
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Reactive Oxygen Species)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 1.11.1.15 (Peroxiredoxin III)
RN  - EC 1.8.1.9 (Thioredoxin Reductase 2)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Blood Glucose
MH  - Cell Line
MH  - Diabetes Mellitus, Experimental/blood/*enzymology
MH  - Diabetic Cardiomyopathies/blood/*enzymology
MH  - Enzyme Repression
MH  - Gene Expression
MH  - Hyperglycemia/blood/*enzymology
MH  - Male
MH  - Oxidative Stress
MH  - Peroxiredoxin III/*physiology
MH  - Protective Factors
MH  - Quercetin/pharmacology
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Thioredoxin Reductase 2/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Cardiac dysfunction
OT  - Diabetic cardiomyopathy
OT  - Oxidative stress
OT  - Peroxiredoxin-3
EDAT- 2016/07/10 06:00
MHDA- 2018/01/05 06:00
CRDT- 2016/07/10 06:00
PHST- 2016/01/18 00:00 [received]
PHST- 2016/06/15 00:00 [revised]
PHST- 2016/06/18 00:00 [accepted]
PHST- 2016/07/10 06:00 [entrez]
PHST- 2016/07/10 06:00 [pubmed]
PHST- 2018/01/05 06:00 [medline]
AID - S0891-5849(16)30307-0 [pii]
AID - 10.1016/j.freeradbiomed.2016.06.019 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2016 Aug;97:489-500. doi: 
      10.1016/j.freeradbiomed.2016.06.019. Epub 2016 Jul 5.