PMID- 27394968
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20170812
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 56
DP  - 2016 Sep
TI  - Effects of dietary oxidized konjac glucomannan sulfates (OKGMS) and 
      acidolysis-oxidized konjac glucomannan (A-OKGM) on the immunity and expression of 
      immune-related genes of Schizothorax prenanti.
PG  - 96-105
LID - S1050-4648(16)30423-5 [pii]
LID - 10.1016/j.fsi.2016.07.003 [doi]
AB  - In the present study, konjac glucomannan (KGM) was degraded by H2O2, and then 
      used trisulfonated sodium amine and HCl, individually, to obtain two kinds of 
      derivatives: oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized 
      konjac glucomannan (A-OKGM). The effects of two OKGM modified products on the 
      immune parameters and expressions of toll-like receptor 22 (TLR22), myeloid 
      differentiation factor 88 (MyD88) and interferon regulatory factors 7 (IRF7) 
      genes in Schizothorax prenanti were determined. The alternative haemolytic 
      complement (ACH50) activity was found to be significantly increased by the OKGMS 
      diets. The immunoglobulin M (IgM) level was significantly enhanced by the OKGMS 
      diets. The lysozyme activity was significantly increased by both OKGMS and A-OKGM 
      diets. The superoxide dismutase (T-SOD) activity in fish fed with all doses of 
      OKGMS diets was significantly higher than that in fish fed with basal diet. The 
      glutathione peroxidase (GSH-PX) activity in fish fed with 0.8% and 1.6% A-OKGM 
      diets was significantly higher than control group. The malondialdehyde (MDA) 
      level was significantly decreased by both OKGMS and A-OKGM diets. The 0.8% A-OKGM 
      diet significantly up-regulated TLR22 gene expression in the head kidney and 
      spleen. TLR22 gene expression was significantly promoted by all OKGMS diets in 
      the mesonephros and liver. The MyD88 mRNA level in 1.6% A-OKGM group 
      significantly increased in the head kidney. The low dose of OKGMS significantly 
      induced the MyD88 gene expression in the mesonephros, gut and liver, while 0.8% 
      A-OKGM group also showed a significantly enhanced MyD88 mRNA expression in the 
      gut. High dose of OKGMS significantly increased the IRF7 mRNA expression in the 
      mesonephros and spleen. Fish fed with low dose of A-OKGM showed significantly 
      higher expression of IRF7 in the gut and liver. Present study suggested that 
      OKGMS and A-OKGM can act as immunostimulant to improve the immune indexes and 
      up-regulate the immune-related gene expressions.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Chen, Mingrui
AU  - Chen M
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Wang, Hongjie
AU  - Wang H
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Yan, Qiuping
AU  - Yan Q
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Zheng, Qiaoran
AU  - Zheng Q
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Yang, Min
AU  - Yang M
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Lv, Zhenzhen
AU  - Lv Z
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - He, Mei
AU  - He M
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Feng, Limei
AU  - Feng L
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Zhao, Jiaqi
AU  - Zhao J
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Tang, Tingting
AU  - Tang T
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China.
FAU - Wu, Yinglong
AU  - Wu Y
AD  - College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, 
      PR China. Electronic address: 405135288@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20160707
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Mannans)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Sulfates)
RN  - 0 (Toll-Like Receptors)
RN  - 36W3E5TAMG ((1-6)-alpha-glucomannan)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animals
MH  - *Cyprinidae/genetics/immunology/metabolism
MH  - Diet/*veterinary
MH  - *Dietary Supplements/analysis
MH  - Dose-Response Relationship, Drug
MH  - Fish Proteins/*genetics/metabolism
MH  - Gene Expression Regulation/*immunology
MH  - Immunity, Innate/*immunology
MH  - Interferon Regulatory Factor-7/genetics/metabolism
MH  - *Mannans
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - Oxidation-Reduction
MH  - Random Allocation
MH  - Sulfates/metabolism
MH  - Toll-Like Receptors/genetics/metabolism
OTO - NOTNLM
OT  - Acidolysis-oxidized konjac glucomannan
OT  - Immunity
OT  - Interferon regulatory factors7
OT  - Myeloid differentiation factor 88
OT  - Oxidized konjac glucomannan sulfates
OT  - Toll-like receptor 22
EDAT- 2016/07/11 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/07/11 06:00
PHST- 2016/04/18 00:00 [received]
PHST- 2016/07/05 00:00 [revised]
PHST- 2016/07/05 00:00 [accepted]
PHST- 2016/07/11 06:00 [entrez]
PHST- 2016/07/11 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - S1050-4648(16)30423-5 [pii]
AID - 10.1016/j.fsi.2016.07.003 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Sep;56:96-105. doi: 10.1016/j.fsi.2016.07.003. Epub 
      2016 Jul 7.