PMID- 27398236
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160711
LR  - 20240325
IS  - 2090-7907 (Print)
IS  - 2090-7907 (Electronic)
IS  - 2090-7907 (Linking)
VI  - 2012
DP  - 2012
TI  - Nucleotide Sequencing and SNP Detection of Toll-Like Receptor-4 Gene in Murrah 
      Buffalo (Bubalus bubalis).
PG  - 659513
LID - 10.5402/2012/659513 [doi]
LID - 659513
AB  - Toll-like receptor-4 (TLR-4) has an important pattern recognition receptor that 
      recognizes endotoxins associated with gram negative bacterial infections. The 
      present investigation was carried out to study nucleotide sequencing and SNP 
      detection by PCR-RFLP analysis of the TLR-4 gene in Murrah buffalo. Genomic DNA 
      was isolated from 102 lactating Murrah buffalo from NDRI herd. The amplified PCR 
      fragments of TLR-4 comprised of exon 1, exon 2, exon 3.1, and exon 3.2 were 
      examined to RFLP. PCR products were obtained with sizes of 165, 300, 478, and 
      409 bp. TLR-4 gene of investigated Murrah buffaloes was highly polymorphic with 
      AA, AB, and BB genotypes as revealed by PCR-RFLP analysis using Dra I, Hae III, 
      and Hinf I REs. Nucleotide sequencing of the amplified fragment of TLR-4 gene of 
      Murrah buffalo was done. Twelve SNPs were identified. Six SNPs were nonsynonymous 
      resulting in change in amino acids. Murrah is an indigenous Buffalo breed and the 
      presence of the nonsynonymous SNP is indicative of its unique genomic 
      architecture. Sequence alignment and homology across species using BLAST analysis 
      revealed 97%, 97%, 99%, 98%, and 80% sequence homology with Bos taurus, Bos 
      indicus, Ovis aries, Capra hircus, and Homo sapiens, respectively.
FAU - Mitra, M
AU  - Mitra M
AD  - Department of Animal Genetics and Breeding, Faculty of Veterinary and Animal 
      Sciences, West Bengal University of Animal and Fishery Sciences, 37768 Kshudiram 
      Bose Sarani, West Bengal, Kolkata 700037, India.
FAU - Taraphder, S
AU  - Taraphder S
AD  - Department of Animal Genetics and Breeding, Faculty of Veterinary and Animal 
      Sciences, West Bengal University of Animal and Fishery Sciences, 37768 Kshudiram 
      Bose Sarani, West Bengal, Kolkata 700037, India.
FAU - Sonawane, G S
AU  - Sonawane GS
AD  - Dairy Cattle Breeding Division, NDRI, Karnal-132001, Haryana, India.
FAU - Verma, A
AU  - Verma A
AD  - Dairy Cattle Breeding Division, NDRI, Karnal-132001, Haryana, India.
LA  - eng
PT  - Journal Article
DEP - 20120329
PL  - United States
TA  - ISRN Mol Biol
JT  - ISRN molecular biology
JID - 101587482
PMC - PMC4908255
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/03/29
CRDT- 2016/07/12 06:00
PHST- 2011/11/22 00:00 [received]
PHST- 2011/12/15 00:00 [accepted]
PHST- 2016/07/12 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/03/29 00:00 [pmc-release]
AID - 10.5402/2012/659513 [doi]
PST - epublish
SO  - ISRN Mol Biol. 2012 Mar 29;2012:659513. doi: 10.5402/2012/659513. eCollection 
      2012.