PMID- 27401487
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20220311
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 11
IP  - 10
DP  - 2017 Oct
TI  - Characterization of layered chondrocyte sheets created in a co-culture system 
      with synoviocytes in a hypoxic environment.
PG  - 2885-2894
LID - 10.1002/term.2192 [doi]
AB  - Endeavouring to repair and regenerate articular cartilage using cell sheets, we 
      have previously established a co-culture system of chondrocytes and synoviocytes, 
      and have reported the successful and rapid production of chondrocyte sheets. In 
      the present study, to examine the effects of oxygen concentration on the 
      chondrocyte sheets, we co-cultured human articular chondrocytes and human 
      synoviocytes in 2%, 5% and 21% oxygen, and measured chondrocyte metabolic 
      activity and proliferation activities under each condition for 14 days in 
      culture. Layered chondrocyte sheets were also created under each condition and 
      the proteoglycan (PG) level was compared with the gene expression of type I 
      collagen (COL1), COL2, COL27, tissue metallopeptidase inhibitor 1 (TIMP1), 
      fibronectin-1 (FN1), SRY-related HMG Box 9 (SOX9), aggrecan-1 (ACAN), 
      integrin-alpha10 (ITGalpha10), matrix metalloproteinase 3 (MMP3), MMP13 and a disintegrin 
      and metalloproteinase with thrombospondin motif 5 (ADAMTS5). Compared with 5% and 
      21% oxygen, the 2% condition caused significantly greater cell metabolic activity 
      and proliferation (p < 0.05). The 2% condition produced a 10% greater PG level 
      compared with 21% oxygen (p < 0.05). All conditions increased the expression of 
      chondrocyte-specific genes, such as COL2, and were associated with low expression 
      levels of catabolic factors, such as MMP3 and MMP13. These observations indicated 
      that the specificity of the chondrocyte sheets was maintained under all 
      conditions. The culture times did not differ between the 5% and 21% conditions. 
      Compared with 21% oxygen, layered chondrocyte sheets rich in extracellular matrix 
      were created 2.85 days earlier in 2% oxygen, which is similar to the level found 
      in deep cartilage. Copyright (c) 2016 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Kokubo, Mami
AU  - Kokubo M
AD  - Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical 
      University, Tokyo, Japan.
FAU - Sato, Masato
AU  - Sato M
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Kanagawa, Japan.
FAU - Yamato, Masayuki
AU  - Yamato M
AD  - Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical 
      University, Tokyo, Japan.
FAU - Mitani, Genya
AU  - Mitani G
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Kanagawa, Japan.
FAU - Uchiyama, Yoshiyasu
AU  - Uchiyama Y
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Kanagawa, Japan.
FAU - Mochida, Joji
AU  - Mochida J
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Kanagawa, Japan.
FAU - Okano, Teruo
AU  - Okano T
AD  - Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical 
      University, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160712
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Hypoxia
MH  - Cell Proliferation
MH  - Chondrocytes/*cytology/metabolism
MH  - Coculture Techniques/*methods
MH  - Collagen Type I/metabolism
MH  - Collagen Type II/metabolism
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Staining and Labeling
MH  - Synoviocytes/*cytology
MH  - Young Adult
OTO - NOTNLM
OT  - human chondrocyte
OT  - hypoxia
OT  - layered chondrocyte sheets
OT  - regeneration
OT  - temperature-responsive culture dishes
EDAT- 2016/07/13 06:00
MHDA- 2018/06/19 06:00
CRDT- 2016/07/13 06:00
PHST- 2015/08/23 00:00 [received]
PHST- 2016/02/02 00:00 [revised]
PHST- 2016/03/14 00:00 [accepted]
PHST- 2016/07/13 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2016/07/13 06:00 [entrez]
AID - 10.1002/term.2192 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2017 Oct;11(10):2885-2894. doi: 10.1002/term.2192. Epub 
      2016 Jul 12.