PMID- 27402219 OWN - NLM STAT- MEDLINE DCOM- 20170227 LR - 20180924 IS - 1532-8198 (Electronic) IS - 1092-9134 (Linking) VI - 23 DP - 2016 Aug TI - Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1. PG - 21-8 LID - S1092-9134(16)30111-3 [pii] LID - 10.1016/j.anndiagpath.2016.05.003 [doi] AB - Follicular dendritic cell sarcoma (FDCS) is a rare low-grade neoplasm with the phenotype of FDC cells. This rare sarcoma has been well known for being mistaken for a variety of neoplasms (mainly meningioma), particularly at extranodal sites. Diagnosis of FDCS mainly relies on characteristic histologic appearance supplemented by immunohistochemistry and electron microscopy. In this study, we reviewed 15 FDCSs retrieved from our consultation files and stained them for newly reported or novel markers (PD-L1, Rb1, MDM2, and somatostatin receptor 2A [SSTR2A]) in addition to conventional FDC markers. Patients were 7 men and 7 women (1 unspecified) with a mean age of 47 years (20-75 years). The tumor site was lymph nodes (6) or spleen (2), both (1) and extranodal sites of head and neck (4) or abdominal cavity (2). Treatment was variable combinations of surgery and aggressive chemotherapy/radiotherapy. Four of 8 patients with follow-up died of disease within 1 to 10 years. All tumors expressed at least 1 FDC marker: CD21 (8/13), CD23 (2/13), CD35 (8/12), CNA.42 (13/14), Clusterin (8/13), Fascin (15/15) and D2-40/podoplanin (7/14). Epstein-Barr virus (EBER-1/2 in situ hybridization) was performed successfully in 10 conventional variants; all were negative. Five of 14 cases (36%) stained strongly for SSTR2A with a distinctive membranous pattern. Residual lymphoid follicles surrounding some of the tumors stained similarly for SSTR2A. Seven (54%) of 13 assessable cases showed moderate to strong membranous staining for PD-L1 in greater than 5% of the neoplastic cells. The Rb1 antigen was lost in 4 (28%) of 14 cases. MDM2 stained less than 5% to 20% of the tumor cells in 5 (36%) of 14 cases; 2 of them showed amplification by fluorescence in situ hybridization (FISH). CDK4 was negative except for weak staining in 1 of 14 cases. This study adds to the existing few clinicopathologic series on FDCS and represents the first study to show MDM2 amplification in this entity. Our results regarding frequent SSTR2A expression in FDCS are novel and might be of potential diagnostic and therapeutic relevance. SSTR2A expression in FDCS represents a further confusing factor when thinking of meningioma which uniformly expresses this receptor. FDCS occurring within the retroperitoneum and/or the abdominal cavity may closely mimic dedifferentiated liposarcoma, particularly if MDM2 positive and/or amplified and should thus be carefully assessed for expression of FDC markers. CI - Copyright (c) 2016 Elsevier Inc. All rights reserved. FAU - Agaimy, Abbas AU - Agaimy A AD - Institute of Pathology, University Hospital, Erlangen, Germany. Electronic address: abbas.agaimy@uk-erlangen.de. FAU - Michal, Michael AU - Michal M AD - Department of Pathology, Charles University, Biomedical Center, Faculty of Medicine in Plzen and Charles University Hospital Plzen, Plzen, Czech Republic. FAU - Hadravsky, Ladislav AU - Hadravsky L AD - Department of Pathology, Faculty of Medicine, Charles University, Plzen, Czech Republic; Department of Pathology, Charles University, 3rd Medical Faculty and Charles University Hospital Royal Vineyards, Prague, Czech Republic. FAU - Michal, Michal AU - Michal M AD - Department of Pathology, Faculty of Medicine, Charles University, Plzen, Czech Republic. LA - eng PT - Journal Article DEP - 20160518 PL - United States TA - Ann Diagn Pathol JT - Annals of diagnostic pathology JID - 9800503 RN - 0 (B7-H1 Antigen) RN - 0 (Biomarkers, Tumor) RN - 0 (CD274 protein, human) RN - 0 (Receptors, Somatostatin) RN - 0 (SSTR2 protein, human) RN - EC 2.3.2.27 (MDM2 protein, human) RN - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2) SB - IM MH - Adult MH - Aged MH - B7-H1 Antigen/*metabolism MH - Biomarkers, Tumor/*analysis MH - Dendritic Cell Sarcoma, Follicular/*diagnosis/metabolism MH - Female MH - Humans MH - Immunohistochemistry/methods MH - In Situ Hybridization, Fluorescence/methods MH - Male MH - Middle Aged MH - Proto-Oncogene Proteins c-mdm2/*metabolism MH - Receptors, Somatostatin/*metabolism MH - Young Adult OTO - NOTNLM OT - FDC tumor OT - Follicular dendritic cell sarcoma OT - Head and neck OT - MDM2 OT - PD-L1 OT - Somatostatin receptor OT - Spleen EDAT- 2016/07/13 06:00 MHDA- 2017/02/28 06:00 CRDT- 2016/07/13 06:00 PHST- 2016/05/10 00:00 [received] PHST- 2016/05/16 00:00 [accepted] PHST- 2016/07/13 06:00 [entrez] PHST- 2016/07/13 06:00 [pubmed] PHST- 2017/02/28 06:00 [medline] AID - S1092-9134(16)30111-3 [pii] AID - 10.1016/j.anndiagpath.2016.05.003 [doi] PST - ppublish SO - Ann Diagn Pathol. 2016 Aug;23:21-8. doi: 10.1016/j.anndiagpath.2016.05.003. Epub 2016 May 18.