PMID- 27404554
OWN - NLM
STAT- MEDLINE
DCOM- 20170811
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 112
DP  - 2016 Jun 22
TI  - Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing 
      Metabolic Phenotypes.
LID - 10.3791/54128 [doi]
LID - 54128
AB  - Immune response results from a complex interplay between the antigen non-specific 
      innate immune system and the antigen specific adaptive immune system. The immune 
      system is a constant balance in maintaining tolerance to self-molecules and 
      reacting rapidly to pathogens. Dendritic cells (DCs) are powerful professional 
      antigen presenting cells that link the innate immune system to the adaptive 
      immune system and balance the adaptive response between self and non-self. 
      Depending on the maturation signals, immature dendritic cells can be selectively 
      stimulated to differentiate into immunogenic or tolerogenic DCs. Immunogenic 
      dendritic cells provide proliferation signals to antigen-specific T cells for 
      clonal expansion; while tolerogenic dendritic cells regulate tolerance by 
      antigen-specific T-cell deletion or clonal expansion of regulatory T-cells. Due 
      to this unique property, dendritic cells are highly sought after as therapeutic 
      agents for cancer and autoimmune diseases. Dendritic cells can be loaded with 
      specific antigens in vitro and injected into the human body to mount a specific 
      immune response both immunogenic and tolerogenic. This work presents a means to 
      generate in vitro from monocytes, immature monocyte derived dendritic cells 
      (moDCs), tolerogenic and mature moDCs that differ in surface marker expression, 
      function and metabolic phenotypes.
FAU - Sim, Wen Jing
AU  - Sim WJ
AD  - Translational Immunology, Institute of Molecular and Cell Biology, Agency for 
      Science, Technology and Research.
FAU - Malinarich, Frano
AU  - Malinarich F
AD  - Translational Immunology, Institute of Molecular and Cell Biology, Agency for 
      Science, Technology and Research.
FAU - Fairhurst, Anna-Marie
AU  - Fairhurst AM
AD  - Singapore Immunology Network.
FAU - Connolly, John Edward
AU  - Connolly JE
AD  - Translational Immunology, Institute of Molecular and Cell Biology, Agency for 
      Science, Technology and Research; Institute of Biomedical Studies, Baylor 
      University; jeconnolly@imcb.a-star.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20160622
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Antigens)
SB  - IM
MH  - Antigens
MH  - *Dendritic Cells
MH  - Humans
MH  - Immune Tolerance
MH  - T-Lymphocytes, Regulatory
PMC - PMC4993244
EDAT- 2016/07/13 06:00
MHDA- 2017/08/12 06:00
PMCR- 2018/06/22
CRDT- 2016/07/13 06:00
PHST- 2016/07/13 06:00 [entrez]
PHST- 2016/07/13 06:00 [pubmed]
PHST- 2017/08/12 06:00 [medline]
PHST- 2018/06/22 00:00 [pmc-release]
AID - 54128 [pii]
AID - 10.3791/54128 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Jun 22;(112):54128. doi: 10.3791/54128.