PMID- 27405171
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20181202
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 51
IP  - 1
DP  - 2016 Jan
TI  - [Simultaneous determination of 11 mycotoxins in malt by isotope internal 
      standard-UPLC-MS/MS].
PG  - 110-5
AB  - A suitable ultra-high performance liquid chromatography coupled with tandem mass 
      spectrometry (UPLC-MS/MS) method was developed for the determination of 11 
      mycotoxins with isotope internal standard in malt. The mycotoxins in malt were 
      extracted and purified by one-step ultrasonic extraction procedure using 
      acetonitrile/water/acetic acid (80 : 19 : 1), and then detected and confirmed by 
      UPLC-MS/MS, and quantified by isotope labeled AFB1 ([13C17]-AFB1) and ZEN 
      ([13C18]-ZEN) internal standards. Rapid separation of the 11 mycotoxins was 
      successfully achieved on a Phenomenex Kinetex C18 column (100 mm x 2.1 mm, 2.6 
      mum) with gradient elution using the mobile phase of methanol containing 0.1% 
      formic acid and 2 mmol x L(-1) ammonium acetate in water. Simultaneous 
      acquisition was performed in multiple reaction monitoring (MRM) mode with 
      electrospray ionization (ESI) source operated in both positive and negative 
      ionization modes. The established method provided a good linearity for the 11 
      mycotoxins within their respective linear ranges with correlation coefficients 
      all higher than 0.999 1. The average recoveries ranged from 75.0% to 117.0% with 
      relative standard deviations (RSDs) below 5.1%. The limits of detection (LODs) 
      and quantitation (LOQs) ranged from 0.05 to 30 mug x kg(-1) and 0.15 to 87.5 mug x 
      kg(-1), respectively, which were below the maximum residue levels (MRLs) set by 
      the European Union. Twenty malt samples were analyzed and nine samples were 
      detected with mycotoxins, which were confirmed according to the same fragment 
      ions found in positive samples and the standards at the same retention time. This 
      study has demonstrated that the one-step extraction procedure of mycotoxins from 
      complex matrices coupled to UPLC-MS/MS method is simple, quick, accurate and 
      sensitive for quantitative and qualitative analysis of multiple mycotoxins in 
      malt.
FAU - Wang, Sha
AU  - Wang S
FAU - Kong, Wei-jun
AU  - Kong WJ
FAU - Yang, Mei-hua
AU  - Yang MH
LA  - chi
PT  - Journal Article
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Mycotoxins)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Fermentation
MH  - Hordeum/*chemistry
MH  - Limit of Detection
MH  - Mycotoxins/analysis/*isolation & purification
MH  - *Tandem Mass Spectrometry
EDAT- 2016/07/14 06:00
MHDA- 2016/07/23 06:00
CRDT- 2016/07/14 06:00
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/14 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
PST - ppublish
SO  - Yao Xue Xue Bao. 2016 Jan;51(1):110-5.